biostudies-literatureDai YFundamental Research Funds for the Central UniversitiesNational Natural Science Foundation of ChinaMinistry of Agriculture48-55https://www.ebi.ac.uk/biostudies/studies/S-EPMC7111660biostudies-literatureUnknown55L-type lectins are involved in glycoproteins secretory pathways and are associated with many immune responses. There is growing evidence that L-type lectins are also involved in viral replication. In this study, a novel L-type lectin (named as PcL-lectin) was identified from red swamp crayfish (Procambarus clakii). Gene sequencing and phylogenetic tree analysis results showed that the PcL-lectin was a kind of endoplasmic reticulum Golgi intermediate compartment-53 (ERGIC-53). The expression level of PcL-lectin was significantly down regulated in crayfish after challenged with white spot syndrome virus (WSSV). Recombinant PcL-lectin protein facilitated the replication of WSSV in crayfish. In addition, WSSV replication was decreased when endogenous PcL-lectin was knocked down by RNA interferFish & shellfish immunologyA novel L-type lectin was required for the multiplication of WSSV in red swamp crayfish (Procambarus clakii).PMC711166031372563315021992015QC019315726572014PY035201401Qin QZhao LQin ZWang YLin LDai YYuan JLan JfalseA novel L-type lectin was required for the multiplication of WSSV in red swamp crayfish (Procambarus clakii).L-type lectins are involved in glycoproteins secretory pathways and are associated with many immune responses. There is growing evidence that L-type lectins are also involved in viral replication. In this study, a novel L-type lectin (named as PcL-lectin) was identified from red swamp crayfish (Procambarus clakii). Gene sequencing and phylogenetic tree analysis results showed that the PcL-lectin was a kind of endoplasmic reticulum Golgi intermediate compartment-53 (ERGIC-53). The expression level of PcL-lectin was significantly down regulated in crayfish after challenged with white spot syndrome virus (WSSV). Recombinant PcL-lectin protein facilitated the replication of WSSV in crayfish. In addition, WSSV replication was decreased when endogenous PcL-lectin was knocked down by RNA interfer2016-01-01T00:00:00Z2016 Aug2025-06-01T12:22:41.039Z2025-06-01T12:22:41.039ZS-EPMC71116602720879310.1016/j.fsi.2016.05.020