biostudies-literatureXu L“Blue Ocean Talent Plan” Innovation and Entrepreneurship TeamNational Natural Science Foundation of ChinaBeijing Technology and Business University560-569https://www.ebi.ac.uk/biostudies/studies/S-EPMC7112149biostudies-literatureUnknown61(4)This article focuses on the establishment of an accurate and sensitive quantitation method for the analysis of furan fatty acids. In particular, the sensitivity of GC/MS and UPLC/ESI/MS/MS was compared for the identification and quantification of furan fatty acids. Different methylation methods were tested with respect to GC/MS analysis. Special attention needs to be paid to the methylation of furan fatty acids, as acidic catalysts might lead to the degradation of the furan ring. GC/MS analysis in full-scan mode demonstrated that the limit of quantitation was 10 μM. UPLC/ESI/MS/MS in multiple reaction monitoring mode displayed a higher detection sensitivity than GC/MS. Moreover, the identification of furan fatty acids with charge-reversal derivatization was tested in the positive mode withJournal of lipid researchDevelopment of a sensitive and quantitative method for the identification of two major furan fatty acids in human plasma.PMC71121492018110700013160139820171049Xu LLiu YYin HHu CLi SWang YVetter WfalseDevelopment of a sensitive and quantitative method for the identification of two major furan fatty acids in human plasma.This article focuses on the establishment of an accurate and sensitive quantitation method for the analysis of furan fatty acids. In particular, the sensitivity of GC/MS and UPLC/ESI/MS/MS was compared for the identification and quantification of furan fatty acids. Different methylation methods were tested with respect to GC/MS analysis. Special attention needs to be paid to the methylation of furan fatty acids, as acidic catalysts might lead to the degradation of the furan ring. GC/MS analysis in full-scan mode demonstrated that the limit of quantitation was 10 μM. UPLC/ESI/MS/MS in multiple reaction monitoring mode displayed a higher detection sensitivity than GC/MS. Moreover, the identification of furan fatty acids with charge-reversal derivatization was tested in the positive mode with2020-01-01T00:00:00Z2020 Apr2026-05-07T10:54:21.248Z2026-04-07T22:47:11.953ZS-EPMC71121493202951210.1194/jlr.d11900051410.1194/jlr.D119000514