biostudies-literatureCarter SDNational Center for Research ResourcesNIDA NIH HHSNCRR NIH HHSNIA NIH HHSHoward Hughes Medical InstituteNIAID NIH HHSLouis V. Gerstner Jr., Scholars ProgramNational Institute of General Medical SciencesNational Institute on AgingAEI/FEDERLeon Levy FoundationEuropean Research CouncilU.S. Department of DefenseIsrael Science FoundationNINDS NIH HHSNCI NIH HHSMinisterio de Economía y CompetitividadNational Institute on Drug AbusePittsburgh FoundationNIGMS NIH HHSNIH HHSNational Science Foundationeaay9572https://www.ebi.ac.uk/biostudies/studies/S-EPMC7112762biostudies-literatureUnknown6(14)The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.Science advancesRibosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.PMC7112762P50 AI150464P41 RR001209R01 AG056387MCB-1408986BIO2016-76400-RS10 RR019003R56 AG058593P30 CA013696K01 AG045335P50GM082545K08 DA031241P41 GM103310R35 GM122588S10 RR0254881S10RR016236S10 RR029300K08DA0312411S10RR0190031285/14S10 OD0162361S10RR025488R01 GM029169R37 GM029169PR141292F30 NS093798AIC-A-2011-0638K01AG045335310649GM29169P50 GM082545Morgenstern TJAslanoglou DWolf SGMitchell WGJensen GJEisenman LCarter SDWills ZPBalla TCalderon MJRice WJPilli JLanglois RLevy ESFrank JFreyberg RJJavitch JAFass DAguilar JIFarino ZJCourel MHampton CMGrassucci RAFreyberg ZAridor MShiva SBartolini FGreenberg NLFish KNVolchuk AWalter PLi WWigge CCarazo JMTran NHMurray SAWallace CTSiegmund SEGeorge EWWatkins SCPemberton JArea-Gomez EMelero RYi EfalseRibosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic β-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.2020-01-01T00:00:00Z2020 Apr2026-05-07T07:26:11.979Z2020-05-22T16:25:17ZS-EPMC71127623227004010.1126/sciadv.aay9572