{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yi L"],"funding":["Ji Lin Province Major Scientific and Technological Achievements Transformation Program","Ji Lin Province Technology and Development Program","Ministry of Agriculture Scientific and Technological Achievements Transformation Program"],"pagination":["281-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7112914"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["179(1)"],"pubmed_abstract":["A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and differentiated the CDV wild-type and vaccine strains by two separate RT-PCRs. T"],"journal":["Journal of virological methods"],"pubmed_title":["Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs."],"pmcid":["PMC7112914"],"funding_grant_id":["20080213","2010GB2B100098","20090531","10ZDZH010"],"pubmed_authors":["Cheng S","Yi L","Xu H","Wang J","Yang S","Luo B","Cheng Y"],"additional_accession":[]},"is_claimable":false,"name":"Development of a combined canine distemper virus specific RT-PCR protocol for the differentiation of infected and vaccinated animals (DIVA) and genetic characterization of the hemagglutinin gene of seven Chinese strains demonstrated in dogs.","description":"A combined reverse-transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (CDV). A pair of primers (P1/P2) was used to detect both CDV wild-type strains and vaccines. Another pair (P3/P4) was used to detect only CDV wild-type strains. A 335bp fragment was amplified from the genomic RNA of the vaccine and wild-type strains. A 555bp fragment was amplified specifically from the genomic RNA of the wild-type strains. No amplification was achieved for the uninfected cells, cells infected with canine parvovirus, canine coronavirus, or canine adenovirus. The combined RT-PCR method detected effectively and differentiated the CDV wild-type and vaccine strains by two separate RT-PCRs. T","dates":{"release":"2012-01-01T00:00:00Z","publication":"2012 Jan","modification":"2025-04-18T18:41:38.538Z","creation":"2020-05-22T15:34:20Z"},"accession":"S-EPMC7112914","cross_references":{"pubmed":["22108430"],"doi":["10.1016/j.jviromet.2011.11.011"]}}