{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["149(1)"],"submitter":["Xiao XL"],"pubmed_abstract":["An outbreak of highly virulent Chinese-type of Porcine Reproductive and Respiratory Syndrome Virus (H-PRRSV) in most areas of China recently has led to huge economic losses and drawn great attention to its diagnosis and disease control. To facilitate rapid identification of H-PRRSV, a fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR for H-PRRSV has been developed. Primers and probe specificity were evaluated with RNA extracted from 5 strains of H-PRRSV and 24 strains of other viruses, the results showed 100% specificity for the selected panel. The assay met the sensitivity of 1 50% tissue culture infective dose (TCID50) per ml of samples from infected pigs. Analysis with 10(5)-1TCID50/ml H-PRRSV samples demonstrated high reproducibility with a coefficient of variation (CV) of 0.5-2.5%. More than two hundred samples from lung, spleen, blood serum specimens obtained from 22 outbreaks of suspected H-PRRS from March to June in 2007 were verified using this assay. The results showed that 68.5% (146 out of 213) of these samples were positive which is 100% consistent with that of the sequencing method. The assay can be performed in less than 3h and thus provide a rapid method for the diagnosis of H-PRRSV as well as for elucidation of the epidemiology of H-PRRSV infections."],"journal":["Journal of virological methods"],"pagination":["49-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7119963"],"repository":["biostudies-literature"],"pubmed_title":["Rapid detection of a highly virulent Chinese-type isolate of Porcine Reproductive and Respiratory Syndrome Virus by real-time reverse transcriptase PCR."],"pmcid":["PMC7119963"],"pubmed_authors":["Cheng BZ","Yang XQ","Chen G","Yu YG","Liu DM","Xiao XL","Li XF","Wu H"],"additional_accession":[]},"is_claimable":false,"name":"Rapid detection of a highly virulent Chinese-type isolate of Porcine Reproductive and Respiratory Syndrome Virus by real-time reverse transcriptase PCR.","description":"An outbreak of highly virulent Chinese-type of Porcine Reproductive and Respiratory Syndrome Virus (H-PRRSV) in most areas of China recently has led to huge economic losses and drawn great attention to its diagnosis and disease control. To facilitate rapid identification of H-PRRSV, a fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase PCR for H-PRRSV has been developed. Primers and probe specificity were evaluated with RNA extracted from 5 strains of H-PRRSV and 24 strains of other viruses, the results showed 100% specificity for the selected panel. The assay met the sensitivity of 1 50% tissue culture infective dose (TCID50) per ml of samples from infected pigs. Analysis with 10(5)-1TCID50/ml H-PRRSV samples demonstrated high reproducibility with a coefficient of variation (CV) of 0.5-2.5%. More than two hundred samples from lung, spleen, blood serum specimens obtained from 22 outbreaks of suspected H-PRRS from March to June in 2007 were verified using this assay. The results showed that 68.5% (146 out of 213) of these samples were positive which is 100% consistent with that of the sequencing method. The assay can be performed in less than 3h and thus provide a rapid method for the diagnosis of H-PRRSV as well as for elucidation of the epidemiology of H-PRRSV infections.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Apr","modification":"2021-02-19T09:19:10Z","creation":"2020-05-22T19:04:03Z"},"accession":"S-EPMC7119963","cross_references":{"pubmed":["18313768"],"doi":["10.1016/j.jviromet.2008.01.009"]}}