{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Kwon KK"],"pubmed_abstract":["Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ<sup>54</sup>)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ<sup>70</sup>) TFs. Here, we show that these unique characteristics of σ<sup>54</sup>-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ<sup>54</sup> TF-based reporter expressio"],"journal":["Scientific reports"],"pagination":["6091"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7142073"],"repository":["biostudies-literature"],"pubmed_title":["Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system."],"pmcid":["PMC7142073"],"pubmed_authors":["Rha E","Kim H","Lee DH","Lee SG","Kwon KK","Lee H","Yeom SJ","Choi SL"],"additional_accession":[]},"is_claimable":false,"name":"Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system.","description":"Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ<sup>54</sup>)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ<sup>70</sup>) TFs. Here, we show that these unique characteristics of σ<sup>54</sup>-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ<sup>54</sup> TF-based reporter expressio","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-06-18T03:48:12.43Z","creation":"2025-05-29T19:03:44.863Z"},"accession":"S-EPMC7142073","cross_references":{"pubmed":["32269250"],"doi":["10.1038/s41598-020-62892-1"]}}