biostudies-literatureUnknown10(1)Kwon KKGenetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ⁵⁴)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ⁷⁰) TFs. Here, we show that these unique characteristics of σ⁵⁴-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ⁵⁴ TF-based reporter expressioScientific reports6091https://www.ebi.ac.uk/biostudies/studies/S-EPMC7142073biostudies-literatureAcclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system.PMC7142073Rha EKim HLee DHLee SGKwon KKLee HYeom SJChoi SLfalseAcclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system.Genetic circuit-based biosensors have emerged as an effective analytical tool in synthetic biology; these biosensors can be applied to high-throughput screening of new biocatalysts and metabolic pathways. Sigma 54 (σ⁵⁴)-dependent transcription factor (TF) can be a valuable component of these biosensors owing to its intrinsic silent property compared to most of the housekeeping sigma 70 (σ⁷⁰) TFs. Here, we show that these unique characteristics of σ⁵⁴-dependent TFs can be used to control the host cell state to be more appropriate for high-throughput screening. The acclimation of cell state was achieved by using guanosine (penta)tetraphosphate ((p)ppGpp)-related genes (relA, spoT) and nutrient conditions, to link the σ⁵⁴ TF-based reporter expressio2020-01-01T00:00:00Z2020 Apr2026-06-18T03:48:12.43Z2025-05-29T19:03:44.863ZS-EPMC71420733226925010.1038/s41598-020-62892-1