{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Inoue K"],"funding":["Grant Agency of the Czech Republic","STAR-UBB Advanced Fellowship-Intern","the Louis and Lyra Richmond Memorial Chair in Life Sciences","Grant-in-Aid for Scientific Research","Academy of Sciences of the Czech Republic","Romanian National Authority for Scientific Research","Japan Science and Technology Agency (JST), PRESTO"],"pagination":["eaaz2441"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7148096"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(15)"],"pubmed_abstract":["Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardarchaeota and from a yet unknown microorganism are expressed in <i>Escherichia coli</i> and mammalian cells, and ion transport assays and patch clamp analyses are used to demonstrate SzR as a novel type of light-driven inward H<sup>+</sup> pump. The mutation of a cytoplasmic glutamate inhibited inward H<sup>+</sup> transport, suggesting that it functions as a cytoplasmic H<sup>+</sup> acceptor. The function, trimeric structure, and H<sup>+</sup> tr"],"journal":["Science advances"],"pubmed_title":["Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H<sup>+</sup> pumps."],"pmcid":["PMC7148096"],"funding_grant_id":["17-04828S","MSM200961801","JPMJPR15P2","JPMJPR1688","PN-III-P4-ID-PCE-2016-0303","17H03007","17-04828 S","15H02391 and 19H04959","18K06109","19H05389, 18H04512, and 18H01837"],"pubmed_authors":["Kandori H","Bulzu PA","Konno M","Beja O","Tomida S","Inoue K","Andrei AS","Tsunoda SP","Hososhima S","Uchihashi T","Ghai R","Banciu HL","Singh M","Watanabe H","Nakamura R"],"additional_accession":[]},"is_claimable":false,"name":"Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H<sup>+</sup> pumps.","description":"Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardarchaeota and from a yet unknown microorganism are expressed in <i>Escherichia coli</i> and mammalian cells, and ion transport assays and patch clamp analyses are used to demonstrate SzR as a novel type of light-driven inward H<sup>+</sup> pump. The mutation of a cytoplasmic glutamate inhibited inward H<sup>+</sup> transport, suggesting that it functions as a cytoplasmic H<sup>+</sup> acceptor. The function, trimeric structure, and H<sup>+</sup> tr","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-05-01T23:53:46.588Z","creation":"2020-05-22T17:27:29Z"},"accession":"S-EPMC7148096","cross_references":{"pubmed":["32300653"],"doi":["10.1126/sciadv.aaz2441"]}}