biostudies-literatureHa HJRadiation Technology R&amp;amp;D program through the National Research Foundation of Korea funded by the Ministry of Science, ICT &amp;amp; Future Planning, Republic of Korea.Radiation Technology R&D program through the National Research Foundation of Korea funded by the Ministry of Science, ICT & Future Planning, Republic of Korea.E387https://www.ebi.ac.uk/biostudies/studies/S-EPMC7154805biostudies-literatureUnknown9(3)Calnexin (CNX) is an integral membrane protein that functions as a chaperone in the endoplasmic reticulum for the correct folding of proteins under stress conditions, rendering organisms tolerant under adverse conditions. Studies have investigated the cytogenetic effects of gamma irradiation (Ɣ-IR) on plants, but information on the molecular response under Ɣ-IR remains limited. Previously, we constructed a cDNA library of an irradiation-sensitive bioindicator plant, <i>Tradescantia</i> BNL4430 (T-4430) under Ɣ-IR, in which the <i>Calnexin-1</i> gene was highly upregulated at 50 mGy treatment. <i>TrCNX1</i> encodes a 61.4 kDa protein with conserved signature motifs similar to already reported <i>CNX1</i>s. <i>TrCNX1</i> expression was evaluated by semiquantitative reverse transcriptase PCR Plants (Basel, Switzerland)Molecular Characterization and Identification of <i>Calnexin 1</i> As a Radiation Biomarker from <i>Tradescantia</i> BNL4430.PMC7154805NRF-2013M2A2A6043621Kang SYHa HJLee GJSubburaj SKim JBKim YSfalseMolecular Characterization and Identification of <i>Calnexin 1</i> As a Radiation Biomarker from <i>Tradescantia</i> BNL4430.Calnexin (CNX) is an integral membrane protein that functions as a chaperone in the endoplasmic reticulum for the correct folding of proteins under stress conditions, rendering organisms tolerant under adverse conditions. Studies have investigated the cytogenetic effects of gamma irradiation (Ɣ-IR) on plants, but information on the molecular response under Ɣ-IR remains limited. Previously, we constructed a cDNA library of an irradiation-sensitive bioindicator plant, <i>Tradescantia</i> BNL4430 (T-4430) under Ɣ-IR, in which the <i>Calnexin-1</i> gene was highly upregulated at 50 mGy treatment. <i>TrCNX1</i> encodes a 61.4 kDa protein with conserved signature motifs similar to already reported <i>CNX1</i>s. <i>TrCNX1</i> expression was evaluated by semiquantitative reverse transcriptase PCR 2020-01-01T00:00:00Z2020 Mar2026-05-02T05:37:34.705Z2020-05-22T17:41:46ZS-EPMC71548053224509410.3390/plants9030387