biostudies-literatureBanerjee RHHS | National Institutes of HealthNIGMS NIH HHSRobert Turrel EndowmentNational Science Foundatione00351-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC7157518biostudies-literatureUnknown11(2)Pathogenicity islands and plasmids bear genes for pathogenesis of various <i>Escherichia coli</i> pathotypes. Although there is a basic understanding of the contribution of these virulence factors to disease, less is known about variation in regulatory networks in determining disease phenotypes. Here, we dissected a regulatory network directed by the conserved iron homeostasis regulator, ferric uptake regulator (Fur), in uropathogenic <i>E. coli</i> (UPEC) strain CFT073. Comparing anaerobic genome-scale Fur DNA binding with Fur-dependent transcript expression and protein levels of the uropathogen to that of commensal <i>E. coli</i> K-12 strain MG1655 showed that the Fur regulon of the core genome is conserved but also includes genes within the pathogenicity/genetic islands. Unexpectedly, rmBioTailoring a Global Iron Regulon to a Uropathogen.PMC7157518R01 GM1158941818303GM108538GM115894P41 GM108538Perna NTKiley PJBanerjee RGlasner JDWelch RASchwartz KJWeisenhorn EMyers KSCoon JJfalseTailoring a Global Iron Regulon to a Uropathogen.Pathogenicity islands and plasmids bear genes for pathogenesis of various <i>Escherichia coli</i> pathotypes. Although there is a basic understanding of the contribution of these virulence factors to disease, less is known about variation in regulatory networks in determining disease phenotypes. Here, we dissected a regulatory network directed by the conserved iron homeostasis regulator, ferric uptake regulator (Fur), in uropathogenic <i>E. coli</i> (UPEC) strain CFT073. Comparing anaerobic genome-scale Fur DNA binding with Fur-dependent transcript expression and protein levels of the uropathogen to that of commensal <i>E. coli</i> K-12 strain MG1655 showed that the Fur regulon of the core genome is conserved but also includes genes within the pathogenicity/genetic islands. Unexpectedly, r2020-01-01T00:00:00Z2020 Mar2026-06-07T07:58:54.421Z2025-06-01T03:54:25.392ZS-EPMC71575183220968210.1128/mBio.00351-20