{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nascimento-Carvalho AC"],"funding":["Sigrid Juséliuksen Säätiö","Fundação de Amparo à Pesquisa do Estado da Bahia","Helsinki University 375-year grant"],"pagination":["88-94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7167785"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(1)"],"pubmed_abstract":["<h4>Aim</h4>To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non-severe community-acquired pneumonia (CAP) in children.<h4>Methods</h4>Patients aged 2-59 months with non-severe CAP (respiratory complaints and radiographic pulmonary infiltrate/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (ClinicalTrials.gov NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92.6%) patients. NPAs were tested for 16 respiratory viruses by PCR. Acute HBoV1 infection was confirmed by measuring specific IgM and IgG responses in paired serum samples.<h4>Results</h4>Respiratory viruses were detected in 693 (91.3%; 95%CI: 89.1-93.2) CAP cases by PCR. HBoV1-DNA was detected in 159 (20.9%; 95%CI: 18.2-24.0) cases. Of these 159 PCR positive cases, acute HBoV1 infection was confirmed serologically in 38 cases (23.9%; 95%CI: 17.8-31.0). Overall, acute HBoV1 infection was confirmed in 5.0% (38/759) of non-severe CAP patients. HBoV1 was detected in 151 cases with at least one other virus making 31.7% of all multiple virus (n = 477) detections. Among all 759 cases, 216 had one respiratory virus detected, and sole HBoV1 was detected in only 8 (3.7%). Acute HBoV1 infection was serologically diagnosed in 34 (22.5%) HBoV1-DNA-positive cases with another virus, compared to 4 (50.0%) cases with sole virus detection (p = 0.09).<h4>Conclusion</h4>HBoV1 was detected by PCR in one fifth of the children with non-severe CAP and acute HBoV1 infection was serologically confirmed in one quarter of these cases."],"journal":["Pediatric pulmonology"],"pubmed_title":["Serologically diagnosed acute human bocavirus 1 infection in childhood community-acquired pneumonia."],"pmcid":["PMC7167785"],"funding_grant_id":["PPSUS No. SUS0023/2013"],"pubmed_authors":["Ruuskanen O","Vuorinen T","Xu M","Nascimento-Carvalho AC","Soderlund-Venermo M","Vilas-Boas AL","Fontoura MH","PNEUMOPAC-Efficacy Study Group","Nascimento-Carvalho CM"],"additional_accession":[]},"is_claimable":false,"name":"Serologically diagnosed acute human bocavirus 1 infection in childhood community-acquired pneumonia.","description":"<h4>Aim</h4>To assess the role of human bocavirus 1 (HBoV1) as a causative agent of non-severe community-acquired pneumonia (CAP) in children.<h4>Methods</h4>Patients aged 2-59 months with non-severe CAP (respiratory complaints and radiographic pulmonary infiltrate/consolidation) attending a University Hospital in Salvador, Brazil were enrolled in a prospective cohort. From 820 recruited children in a clinical trial (ClinicalTrials.gov NCT01200706), nasopharyngeal aspirate (NPA), and acute and convalescent serum samples were obtained from 759 (92.6%) patients. NPAs were tested for 16 respiratory viruses by PCR. Acute HBoV1 infection was confirmed by measuring specific IgM and IgG responses in paired serum samples.<h4>Results</h4>Respiratory viruses were detected in 693 (91.3%; 95%CI: 89.1-93.2) CAP cases by PCR. HBoV1-DNA was detected in 159 (20.9%; 95%CI: 18.2-24.0) cases. Of these 159 PCR positive cases, acute HBoV1 infection was confirmed serologically in 38 cases (23.9%; 95%CI: 17.8-31.0). Overall, acute HBoV1 infection was confirmed in 5.0% (38/759) of non-severe CAP patients. HBoV1 was detected in 151 cases with at least one other virus making 31.7% of all multiple virus (n = 477) detections. Among all 759 cases, 216 had one respiratory virus detected, and sole HBoV1 was detected in only 8 (3.7%). Acute HBoV1 infection was serologically diagnosed in 34 (22.5%) HBoV1-DNA-positive cases with another virus, compared to 4 (50.0%) cases with sole virus detection (p = 0.09).<h4>Conclusion</h4>HBoV1 was detected by PCR in one fifth of the children with non-severe CAP and acute HBoV1 infection was serologically confirmed in one quarter of these cases.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jan","modification":"2024-12-03T15:35:51.463Z","creation":"2020-05-22T17:33:14Z"},"accession":"S-EPMC7167785","cross_references":{"pubmed":["29028159"],"doi":["10.1002/ppul.23891"]}}