{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou Y"],"funding":["Fundamental Research Funds for the Central Universities","Department of S&T of Zhejiang Province","Zhejiang A&F University","Department of Education of Zhejiang Province","National Key Research and Development Program","Agricultural Technology Extension Funds of Zhejiang University","Dabei Agricultural Discipline Development and Talent Training Fund"],"pagination":["83-92"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7173071"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["228"],"pubmed_abstract":["The effects of route of administration on systemic and gut mucosal immune responses induced by porcine epidemic diarrhea virus (PEDV) infection in suckling pigs were investigated. Twenty-four conventional 5-day-old suckling piglets were randomly divided into four groups and were inoculated orally, intranasally (I.N.), intramuscularly (I.M.) with PEDV or DMEM (mock). Pigs were monitored daily for clinical signs and fecal viral load. Blood samples were collected at 7, 14, 21 days post infection (dpi) and subjected for the analyses of serum antibody production, T cell and natural killer (NK) cell frequencies, NK cytotoxicity and serum cytokine levels. Oral inoculation led to higher levels of PEDV-specific IgA antibodies in both serum and gut mucosal sites than did other routes of inoculation."],"journal":["Veterinary microbiology"],"pubmed_title":["Effect of route of inoculation on innate and adaptive immune responses to porcine epidemic diarrhea virus infection in suckling pigs."],"pmcid":["PMC7173071"],"funding_grant_id":["2018C02028","2018FZA6014","2017ZDNT004","2016FR026","Y201635576","2018YFD0701001"],"pubmed_authors":["Zheng J","Liu Y","Li X","Liu Z","Shan Y","Fang W","Wang T","Chen Y","Luo H","Zhou Y","Chen C"],"additional_accession":[]},"is_claimable":false,"name":"Effect of route of inoculation on innate and adaptive immune responses to porcine epidemic diarrhea virus infection in suckling pigs.","description":"The effects of route of administration on systemic and gut mucosal immune responses induced by porcine epidemic diarrhea virus (PEDV) infection in suckling pigs were investigated. Twenty-four conventional 5-day-old suckling piglets were randomly divided into four groups and were inoculated orally, intranasally (I.N.), intramuscularly (I.M.) with PEDV or DMEM (mock). Pigs were monitored daily for clinical signs and fecal viral load. Blood samples were collected at 7, 14, 21 days post infection (dpi) and subjected for the analyses of serum antibody production, T cell and natural killer (NK) cell frequencies, NK cytotoxicity and serum cytokine levels. Oral inoculation led to higher levels of PEDV-specific IgA antibodies in both serum and gut mucosal sites than did other routes of inoculation.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jan","modification":"2026-05-01T23:08:44.056Z","creation":"2025-05-29T21:15:16.307Z"},"accession":"S-EPMC7173071","cross_references":{"pubmed":["30593385"],"doi":["10.1016/j.vetmic.2018.11.019"]}}