biostudies-literatureUnknown26(2)Lee JY<h4>Background/aims</h4>To determine whether the expression of tight junction proteins (TJPs) differs depending on the subtype of functional dyspepsia (FD) and sex.<h4>Methods</h4>Control (n = 95) and FD (n = 165) groups based on Rome III criteria were prospectively enrolled. Gastric mucosal mRNA expression levels of various TJPs (claudins [CLDN] 1, 2, and 4; zonula occludens-1; occludin [OCLN]) were assessed by reverse transcription polymerase chain reaction. Western blot was performed to determine the levels of various TJPs. <i>Helicobacter pylori</i> infection status was evaluated by histology, rapid urease test, and culture. Questionnaires were analyzed.<h4>Results</h4>In all groups irrespective of <i>H. pylori</i> , FD group showed significantly higher CLDN2 mRNA levels than control group (<i>P</i> = 0.048). The level of CLDN4 mRNA expression was significantly lower in female FD group than in male FD group (<i>P</i> = 0.018). In <i>H. pylori</i> uninfected subjects, the level of CLDN1 mRNA expression in female FD group was significantly lower than that of male FD group (<i>P</i> = 0.014). The level of CLDN2 mRNA expression was significantly higher in the male postprandial distress syndrome (<i>P</i> = 0.001) and male epigastric pain syndrome (<i>P</i> = 0.023) groups than in the male control group. In Western blot analysis, the expression of OCLN was significantly elevated 48 hour after the culture with <i>H. pylori</i> strain 43504.<h4>Conclusions</h4><i>H. pylori</i> can affect a variety of TJPs, particularly claudin-4 and occludin. Claudin-2 is thought to be involved in FD irrespective of <i>H. pylori</i> status, especially in the pathophysiology of male FD.Journal of neurogastroenterology and motility248-258https://www.ebi.ac.uk/biostudies/studies/S-EPMC7176499biostudies-literatureExpression of Tight Junction Proteins According to Functional Dyspepsia Subtype and Sex.PMC7176499Ashktorab HSmoot DTPark JHLee DHChoi YJLee JYKim NfalseExpression of Tight Junction Proteins According to Functional Dyspepsia Subtype and Sex.<h4>Background/aims</h4>To determine whether the expression of tight junction proteins (TJPs) differs depending on the subtype of functional dyspepsia (FD) and sex.<h4>Methods</h4>Control (n = 95) and FD (n = 165) groups based on Rome III criteria were prospectively enrolled. Gastric mucosal mRNA expression levels of various TJPs (claudins [CLDN] 1, 2, and 4; zonula occludens-1; occludin [OCLN]) were assessed by reverse transcription polymerase chain reaction. Western blot was performed to determine the levels of various TJPs. <i>Helicobacter pylori</i> infection status was evaluated by histology, rapid urease test, and culture. Questionnaires were analyzed.<h4>Results</h4>In all groups irrespective of <i>H. pylori</i> , FD group showed significantly higher CLDN2 mRNA levels than control group (<i>P</i> = 0.048). The level of CLDN4 mRNA expression was significantly lower in female FD group than in male FD group (<i>P</i> = 0.018). In <i>H. pylori</i> uninfected subjects, the level of CLDN1 mRNA expression in female FD group was significantly lower than that of male FD group (<i>P</i> = 0.014). The level of CLDN2 mRNA expression was significantly higher in the male postprandial distress syndrome (<i>P</i> = 0.001) and male epigastric pain syndrome (<i>P</i> = 0.023) groups than in the male control group. In Western blot analysis, the expression of OCLN was significantly elevated 48 hour after the culture with <i>H. pylori</i> strain 43504.<h4>Conclusions</h4><i>H. pylori</i> can affect a variety of TJPs, particularly claudin-4 and occludin. Claudin-2 is thought to be involved in FD irrespective of <i>H. pylori</i> status, especially in the pathophysiology of male FD.2020-01-01T00:00:00Z2020 Apr2024-10-19T11:28:21.326Z2020-05-22T18:42:12ZS-EPMC71764993223503210.5056/jnm19208