{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kobayashi S"],"funding":["MEXT | Japan Society for the Promotion of Science","Takeda Science Foundation","Japan Agency for Medical Research and Development","JSPS and CAS under the Japan-Czech Research Cooperative Program"],"pagination":["7168"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7189269"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["West Nile virus (WNV) is an important cause of viral encephalitis in birds and animals, including humans. Amino acid 159 of the envelope (E) protein is reportedly implicated in the different levels of neurovirulence in mice infected with WNV NY99 or Eg101. We investigated the role of amino acid 159 of the E protein in the pathogenesis of WNV infection. We produced recombinant WNV with the structural proteins of the NY99 or Eg101 strain (NY-WT or EgCME-WT) and mutant viruses with substitutions of amino acid 159 of the E protein (NY-E-V159I or EgCME-E-I159V). The NY-WT and NY-E-V159I or EgCME-WT and EgCME-E-I159V titers in culture supernatant were similar. The mortality rate and viral titer in the brains of mice inoculated intraperitoneally with NY-WT or NY-E-V159I were also similar. In cont"],"journal":["Scientific reports"],"pubmed_title":["Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection."],"pmcid":["PMC7189269"],"funding_grant_id":["26660220","16K15032","16H06429","18fk0108036h0002","18K14574","19fk0108036h0003","15K19069","17H03910","16H06431","16K21723"],"pubmed_authors":["Sawa H","Yoshii K","Kariwa H","Kobayashi S","Hasebe R","Kaneko C","Kawakami R"],"additional_accession":[]},"is_claimable":false,"name":"Amino acid 159 of the envelope protein affects viral replication and T-cell infiltration by West Nile virus in intracranial infection.","description":"West Nile virus (WNV) is an important cause of viral encephalitis in birds and animals, including humans. Amino acid 159 of the envelope (E) protein is reportedly implicated in the different levels of neurovirulence in mice infected with WNV NY99 or Eg101. We investigated the role of amino acid 159 of the E protein in the pathogenesis of WNV infection. We produced recombinant WNV with the structural proteins of the NY99 or Eg101 strain (NY-WT or EgCME-WT) and mutant viruses with substitutions of amino acid 159 of the E protein (NY-E-V159I or EgCME-E-I159V). The NY-WT and NY-E-V159I or EgCME-WT and EgCME-E-I159V titers in culture supernatant were similar. The mortality rate and viral titer in the brains of mice inoculated intraperitoneally with NY-WT or NY-E-V159I were also similar. In cont","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2025-04-26T14:19:28.97Z","creation":"2020-05-22T19:10:12Z"},"accession":"S-EPMC7189269","cross_references":{"pubmed":["32346055"],"doi":["10.1038/s41598-020-64199-7"]}}