{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhu M"],"funding":["Natural Science Foundation of China","NIEHS NIH HHS","NIEHS","Superfund Program"],"pagination":["155-165"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7198451"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1058"],"pubmed_abstract":["Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play many crucial physiological roles in organisms. Their abnormal levels can cause and indicate various diseases. In the present study, a small-molecule fluorescent probe 2-(imidazo[1,2-a]pyridin-2-yl)phenyl acrylate (IPPA) was designed, synthesized and characterized by NMR, FT-IR and HRMS. IPPA can selectively detect Cys over other analytes because of an approximately 76 times enhancement in fluorescence intensity. The limit of detection of IPPA for Cys was 0.33 μM. The pseudo-first-order rate constant of the reaction between IPPA and Cys was approximately 10 times that of the reaction between IPPA and Hcy (K<sub>Cys</sub> 3.18 × 10<sup>-3</sup> S<sup>-1</sup>vs K<sub>Hcy</sub> 4.92 × 10<sup>-4</sup> S<sup>-1</sup>), indicating tha"],"journal":["Analytica chimica acta"],"pubmed_title":["A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish."],"pmcid":["PMC7198451"],"funding_grant_id":["R01 ES002710","31601657","P42 ES004699","21602043"],"pubmed_authors":["Zhu M","Na R","Li QX","Wang Y","Wang L","Wu X","Hammock BD"],"additional_accession":[]},"is_claimable":false,"name":"A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish.","description":"Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play many crucial physiological roles in organisms. Their abnormal levels can cause and indicate various diseases. In the present study, a small-molecule fluorescent probe 2-(imidazo[1,2-a]pyridin-2-yl)phenyl acrylate (IPPA) was designed, synthesized and characterized by NMR, FT-IR and HRMS. IPPA can selectively detect Cys over other analytes because of an approximately 76 times enhancement in fluorescence intensity. The limit of detection of IPPA for Cys was 0.33 μM. The pseudo-first-order rate constant of the reaction between IPPA and Cys was approximately 10 times that of the reaction between IPPA and Hcy (K<sub>Cys</sub> 3.18 × 10<sup>-3</sup> S<sup>-1</sup>vs K<sub>Hcy</sub> 4.92 × 10<sup>-4</sup> S<sup>-1</sup>), indicating tha","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Jun","modification":"2025-04-29T10:55:59.974Z","creation":"2020-06-16T07:18:19Z"},"accession":"S-EPMC7198451","cross_references":{"pubmed":["30851849"],"doi":["10.1016/j.aca.2019.01.023"]}}