{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(1)"],"submitter":["Reeve MA"],"funding":["Crop Health and Protection Limited"],"pubmed_abstract":["Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry can be used for the characterization and identification of filamentous fungi, for which it is desirable to have a means of growth in which the resulting spectra remain as consistent as possible over time. To this end, we initially opted for growth in oil-overlaid small-volume liquid culture, using a medium (Czapek Dox) not containing significant amount of proteins or peptides, and with protein extraction from the entire culture volume. For both 3-week and 10-day time courses, however, we observed marked spectral changes over growth time, along with lower peak richness compared to agar-plate controls. Guided by the above, we next employed a more nutrient-rich MALDI-TOF MS-compatible liquid-culture medium, now u"],"journal":["Biology methods & protocols"],"pagination":["bpz003"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7200990"],"repository":["biostudies-literature"],"pubmed_title":["A method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times."],"pmcid":["PMC7200990"],"pubmed_authors":["Bachmann D","Reeve MA"],"additional_accession":[]},"is_claimable":false,"name":"A method for filamentous fungal growth and sample preparation aimed at more consistent MALDI-TOF MS spectra despite variations in growth rates and/or incubation times.","description":"Matrix-assisted laser-desorption and ionization time-of-flight mass spectrometry can be used for the characterization and identification of filamentous fungi, for which it is desirable to have a means of growth in which the resulting spectra remain as consistent as possible over time. To this end, we initially opted for growth in oil-overlaid small-volume liquid culture, using a medium (Czapek Dox) not containing significant amount of proteins or peptides, and with protein extraction from the entire culture volume. For both 3-week and 10-day time courses, however, we observed marked spectral changes over growth time, along with lower peak richness compared to agar-plate controls. Guided by the above, we next employed a more nutrient-rich MALDI-TOF MS-compatible liquid-culture medium, now u","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2025-04-07T12:26:17.67Z","creation":"2020-05-22T19:58:35Z"},"accession":"S-EPMC7200990","cross_references":{"pubmed":["32395622"],"doi":["10.1093/biomethods/bpz003"]}}