{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin P"],"funding":["Zhejiang Health Commission"],"pagination":["5373562"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7204090"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2020"],"pubmed_abstract":["<h4>Objective</h4>This research is aimed at determining the vascular health characteristics of carotenoids by evaluating their effect on excessive inflammatory response in endothelial and monocyte cells, the main factors of atherosclerosis.<h4>Methods</h4>Human umbilical vein endothelial cells (HUVECs) or U937 monocytes were treated with escalating concentrations (0.1, 0.5, and 1 <i>μ</i>M) of five most common carotenoids in human plasma, i.e., <i>α</i>-carotene, <i>β</i>-carotene, <i>β</i>-cryptoxanthin, lutein, and lycopene prior to stimulation with 2 mM fructose. We examined the monocyte adhesion to endothelial cells (ECs) and relevant endothelial adhesion molecules. Chemokine and proinflammatory cytokine production as well as intracellular oxidative stress were also assessed in fructose-stimulated ECs and monocytes.<h4>Results</h4>Carotenoids repressed monocyte adhesion to fructose-stimulated ECs dose dependently via decreasing primarily the expression of endothelial VCAM-1. In ECs and monocytes, three carotenoids, i.e., <i>β</i>-cryptoxanthin, lutein, and lycopene, suppressed the fructose-induced expression of chemokines MCP-1, M-CSF, and CXCL-10 and inflammatory cytokines TNF-<i>α</i> and IL-1<i>β</i>, with CXCL-10 being the most repressed inflammatory mediator. <i>β</i>-Cryptoxanthin, lutein, and lycopene dramatically downregulated the fructose-induced CXCL-10 expression in vascular cells. The reduction in the inflammatory response was associated with a slight but significant decrease of intracellular oxidative stress.<h4>Conclusions</h4>Our results show that carotenoids have a variety of anti-inflammatory and antiatherosclerosis activities, which can help prevent or reduce fructose-induced inflammatory vascular diseases."],"journal":["Mediators of inflammation"],"pubmed_title":["Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes."],"pmcid":["PMC7204090"],"funding_grant_id":["2018Z05","2018KY593"],"pubmed_authors":["Lin P","Wu J","Wang Q","Ren Q"],"additional_accession":[]},"is_claimable":false,"name":"Carotenoids Inhibit Fructose-Induced Inflammatory Response in Human Endothelial Cells and Monocytes.","description":"<h4>Objective</h4>This research is aimed at determining the vascular health characteristics of carotenoids by evaluating their effect on excessive inflammatory response in endothelial and monocyte cells, the main factors of atherosclerosis.<h4>Methods</h4>Human umbilical vein endothelial cells (HUVECs) or U937 monocytes were treated with escalating concentrations (0.1, 0.5, and 1 <i>μ</i>M) of five most common carotenoids in human plasma, i.e., <i>α</i>-carotene, <i>β</i>-carotene, <i>β</i>-cryptoxanthin, lutein, and lycopene prior to stimulation with 2 mM fructose. We examined the monocyte adhesion to endothelial cells (ECs) and relevant endothelial adhesion molecules. Chemokine and proinflammatory cytokine production as well as intracellular oxidative stress were also assessed in fructose-stimulated ECs and monocytes.<h4>Results</h4>Carotenoids repressed monocyte adhesion to fructose-stimulated ECs dose dependently via decreasing primarily the expression of endothelial VCAM-1. In ECs and monocytes, three carotenoids, i.e., <i>β</i>-cryptoxanthin, lutein, and lycopene, suppressed the fructose-induced expression of chemokines MCP-1, M-CSF, and CXCL-10 and inflammatory cytokines TNF-<i>α</i> and IL-1<i>β</i>, with CXCL-10 being the most repressed inflammatory mediator. <i>β</i>-Cryptoxanthin, lutein, and lycopene dramatically downregulated the fructose-induced CXCL-10 expression in vascular cells. The reduction in the inflammatory response was associated with a slight but significant decrease of intracellular oxidative stress.<h4>Conclusions</h4>Our results show that carotenoids have a variety of anti-inflammatory and antiatherosclerosis activities, which can help prevent or reduce fructose-induced inflammatory vascular diseases.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2024-02-15T06:06:02.082Z","creation":"2020-05-22T20:23:19Z"},"accession":"S-EPMC7204090","cross_references":{"pubmed":["32410856"],"doi":["10.1155/2020/5373562"]}}