{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liang H"],"funding":["National Natural Science Foundation of China","National Institutes of Health","NIH HHS"],"pagination":["61"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7206796"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(1)"],"pubmed_abstract":["<h4>Background</h4>The disruption of the blood-brain barrier (BBB) plays a critical event in the pathogenesis of ischemia stroke. TGR5 is recognized as a potential target for the treatment for neurologic disorders.<h4>Methods</h4>This study investigated the roles of TGR5 activation in attenuating BBB damage and underlying mechanisms after middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were subjected to model of MCAO and TGR5 agonist, INT777, was administered intranasally. Small interfering RNA (siRNA) for TGR5 and BRCA1 were administered through intracerebroventricular injection 48 h before MCAO. Infarct volumes, brain water content, BBB permeability, neurological scores, Western blot, immunofluorescence staining and co- immunoprecipitation were evaluated.<h4>Results</h4>Endo"],"journal":["Journal of biomedical science"],"pubmed_title":["Activation of TGR5 protects blood brain barrier via the BRCA1/Sirt1 pathway after middle cerebral artery occlusion in rats."],"pmcid":["PMC7206796"],"funding_grant_id":["NS081740","NS082184","81671143"],"pubmed_authors":["McBride DW","Tang J","Liang H","Zhang JH","Matei N","Luo B","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Activation of TGR5 protects blood brain barrier via the BRCA1/Sirt1 pathway after middle cerebral artery occlusion in rats.","description":"<h4>Background</h4>The disruption of the blood-brain barrier (BBB) plays a critical event in the pathogenesis of ischemia stroke. TGR5 is recognized as a potential target for the treatment for neurologic disorders.<h4>Methods</h4>This study investigated the roles of TGR5 activation in attenuating BBB damage and underlying mechanisms after middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were subjected to model of MCAO and TGR5 agonist, INT777, was administered intranasally. Small interfering RNA (siRNA) for TGR5 and BRCA1 were administered through intracerebroventricular injection 48 h before MCAO. Infarct volumes, brain water content, BBB permeability, neurological scores, Western blot, immunofluorescence staining and co- immunoprecipitation were evaluated.<h4>Results</h4>Endo","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 May","modification":"2025-04-22T00:53:09.154Z","creation":"2020-05-22T20:09:10Z"},"accession":"S-EPMC7206796","cross_references":{"pubmed":["32381096"],"doi":["10.1186/s12929-020-00656-9"]}}