biostudies-literatureLiang HNational Natural Science Foundation of ChinaNational Institutes of HealthNIH HHS61https://www.ebi.ac.uk/biostudies/studies/S-EPMC7206796biostudies-literatureUnknown27(1)<h4>Background</h4>The disruption of the blood-brain barrier (BBB) plays a critical event in the pathogenesis of ischemia stroke. TGR5 is recognized as a potential target for the treatment for neurologic disorders.<h4>Methods</h4>This study investigated the roles of TGR5 activation in attenuating BBB damage and underlying mechanisms after middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were subjected to model of MCAO and TGR5 agonist, INT777, was administered intranasally. Small interfering RNA (siRNA) for TGR5 and BRCA1 were administered through intracerebroventricular injection 48 h before MCAO. Infarct volumes, brain water content, BBB permeability, neurological scores, Western blot, immunofluorescence staining and co- immunoprecipitation were evaluated.<h4>Results</h4>EndoJournal of biomedical scienceActivation of TGR5 protects blood brain barrier via the BRCA1/Sirt1 pathway after middle cerebral artery occlusion in rats.PMC7206796NS081740NS08218481671143McBride DWTang JLiang HZhang JHMatei NLuo BXu YfalseActivation of TGR5 protects blood brain barrier via the BRCA1/Sirt1 pathway after middle cerebral artery occlusion in rats.<h4>Background</h4>The disruption of the blood-brain barrier (BBB) plays a critical event in the pathogenesis of ischemia stroke. TGR5 is recognized as a potential target for the treatment for neurologic disorders.<h4>Methods</h4>This study investigated the roles of TGR5 activation in attenuating BBB damage and underlying mechanisms after middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were subjected to model of MCAO and TGR5 agonist, INT777, was administered intranasally. Small interfering RNA (siRNA) for TGR5 and BRCA1 were administered through intracerebroventricular injection 48 h before MCAO. Infarct volumes, brain water content, BBB permeability, neurological scores, Western blot, immunofluorescence staining and co- immunoprecipitation were evaluated.<h4>Results</h4>Endo2020-01-01T00:00:00Z2020 May2025-04-22T00:53:09.154Z2020-05-22T20:09:10ZS-EPMC72067963238109610.1186/s12929-020-00656-9