biostudies-literatureEbrahimpour-Malekshah RShahid Beheshti University of Medical SciencesNIDDK NIH HHSe001033https://www.ebi.ac.uk/biostudies/studies/S-EPMC7206914biostudies-literatureUnknown8(1)<h4>Objective</h4>We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).<h4>Research design and methods</h4>We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm², energy density: 0.2 J/cm²) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 receBMJ open diabetes research & careCombined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus.PMC7206914R43 DK105692R44 DK10569214601Zare FBayat MGhoreishi SKAmini AHashemi SMDeravi NRahmanian MEbrahimpour-Malekshah RChien SMostafavinia AShafikhani SAhmadi HDavoody SHabibi MBayat SfalseCombined therapy of photobiomodulation and adipose-derived stem cells synergistically improve healing in an ischemic, infected and delayed healing wound model in rats with type 1 diabetes mellitus.<h4>Objective</h4>We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).<h4>Research design and methods</h4>We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm², energy density: 0.2 J/cm²) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 rece2020-01-01T00:00:00Z2020 Feb2026-07-16T03:21:48.9Z2026-07-09T10:32:12.077ZS-EPMC72069143209889810.1136/bmjdrc-2019-001033