{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rodrigues JC"],"funding":["Fundação de Amparo à Pesquisa do Estado de São Paulo"],"pagination":["E2692"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7215896"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(8)"],"pubmed_abstract":["Noise exposure represents the second most common cause of acquired sensorineural hearing loss and we observed that tumor necrosis factor α (TNFα) was involved in this context. The effect of <i>Tnfα</i> gene silencing on the expression profile related to the TNFα metabolic pathway in an experimental model of noise-induced hearing loss had not previously been studied.<h4>Methods</h4>Single ears of Wistar rats were pretreated with <i>Tnfα</i> small interfering RNA (siRNA) by trans-tympanic administration 24 h before they were exposed to white noise (120 dBSPL for three hours). After 24 h of noise exposure, we analyzed the electrophysiological threshold and the amplitude of waves I, II, III, and IV in the auditory brain response click. In addition, qRT-PCR was performed to evaluate the TNFα me"],"journal":["International journal of molecular sciences"],"pubmed_title":["New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss."],"pmcid":["PMC7215896"],"funding_grant_id":["14/00405-3"],"pubmed_authors":["de Brito R","do Amaral JB","Silva GAV","Lezirovitz K","Bachi ALL","Rossi M","Rodrigues JC"],"additional_accession":[]},"is_claimable":false,"name":"New Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss.","description":"Noise exposure represents the second most common cause of acquired sensorineural hearing loss and we observed that tumor necrosis factor α (TNFα) was involved in this context. The effect of <i>Tnfα</i> gene silencing on the expression profile related to the TNFα metabolic pathway in an experimental model of noise-induced hearing loss had not previously been studied.<h4>Methods</h4>Single ears of Wistar rats were pretreated with <i>Tnfα</i> small interfering RNA (siRNA) by trans-tympanic administration 24 h before they were exposed to white noise (120 dBSPL for three hours). After 24 h of noise exposure, we analyzed the electrophysiological threshold and the amplitude of waves I, II, III, and IV in the auditory brain response click. In addition, qRT-PCR was performed to evaluate the TNFα me","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-05-07T06:34:08.344Z","creation":"2020-05-26T07:07:18Z"},"accession":"S-EPMC7215896","cross_references":{"pubmed":["32294929"],"doi":["10.3390/ijms21082692"]}}