biostudies-literatureRodrigues JCFundação de Amparo à Pesquisa do Estado de São PauloE2692https://www.ebi.ac.uk/biostudies/studies/S-EPMC7215896biostudies-literatureUnknown21(8)Noise exposure represents the second most common cause of acquired sensorineural hearing loss and we observed that tumor necrosis factor α (TNFα) was involved in this context. The effect of <i>Tnfα</i> gene silencing on the expression profile related to the TNFα metabolic pathway in an experimental model of noise-induced hearing loss had not previously been studied.<h4>Methods</h4>Single ears of Wistar rats were pretreated with <i>Tnfα</i> small interfering RNA (siRNA) by trans-tympanic administration 24 h before they were exposed to white noise (120 dBSPL for three hours). After 24 h of noise exposure, we analyzed the electrophysiological threshold and the amplitude of waves I, II, III, and IV in the auditory brain response click. In addition, qRT-PCR was performed to evaluate the TNFα meInternational journal of molecular sciencesNew Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss.PMC721589614/00405-3de Brito Rdo Amaral JBSilva GAVLezirovitz KBachi ALLRossi MRodrigues JCfalseNew Insights on the Effect of TNF Alpha Blockade by Gene Silencing in Noise-Induced Hearing Loss.Noise exposure represents the second most common cause of acquired sensorineural hearing loss and we observed that tumor necrosis factor α (TNFα) was involved in this context. The effect of <i>Tnfα</i> gene silencing on the expression profile related to the TNFα metabolic pathway in an experimental model of noise-induced hearing loss had not previously been studied.<h4>Methods</h4>Single ears of Wistar rats were pretreated with <i>Tnfα</i> small interfering RNA (siRNA) by trans-tympanic administration 24 h before they were exposed to white noise (120 dBSPL for three hours). After 24 h of noise exposure, we analyzed the electrophysiological threshold and the amplitude of waves I, II, III, and IV in the auditory brain response click. In addition, qRT-PCR was performed to evaluate the TNFα me2020-01-01T00:00:00Z2020 Apr2026-05-07T06:34:08.344Z2020-05-26T07:07:18ZS-EPMC72158963229492910.3390/ijms21082692