{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matousek J"],"funding":["Grantová Agentura České Republiky","Deutsche Forschungsgemeinschaft","Czech Science Foundation"],"pagination":["E3029"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7216239"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(8)"],"pubmed_abstract":["Some viroids-single-stranded, non-coding, circular RNA parasites of plants-are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of <i>Nicotiana tabacum</i> by RNA deep sequencing and molecular methods using infected and transformed tobacco pollen tissues at different developmental stages. AFCVd was not transferable from pollen to seeds in reciprocal pollinations, due to a complete viroid eradication during the last steps of pollen development and fertilization. In pollen, the viroid replication pathway proceeds with detectable replication intermediates, but is dramatically depressed in comparison to leaves. Specific and unspeci"],"journal":["International journal of molecular sciences"],"pubmed_title":["Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes."],"pmcid":["PMC7216239"],"funding_grant_id":["STE 465","17-23183S","18-10515J"],"pubmed_authors":["Matousek J","Mishra AK","Honys D","Drabkova LZ","Kocabek T","Steinbachova L","Potesil D","Steger G"],"additional_accession":[]},"is_claimable":false,"name":"Elimination of Viroids from Tobacco Pollen Involves a Decrease in Propagation Rate and an Increase of the Degradation Processes.","description":"Some viroids-single-stranded, non-coding, circular RNA parasites of plants-are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of <i>Nicotiana tabacum</i> by RNA deep sequencing and molecular methods using infected and transformed tobacco pollen tissues at different developmental stages. AFCVd was not transferable from pollen to seeds in reciprocal pollinations, due to a complete viroid eradication during the last steps of pollen development and fertilization. In pollen, the viroid replication pathway proceeds with detectable replication intermediates, but is dramatically depressed in comparison to leaves. Specific and unspeci","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-04-28T23:55:15.503Z","creation":"2020-05-26T07:07:44Z"},"accession":"S-EPMC7216239","cross_references":{"pubmed":["32344786"],"doi":["10.3390/ijms21083029"]}}