biostudies-literatureUnknown26Bao N<h4>Background</h4>Conopeptides are neuropharmacological peptides derived from the venomous salivary glands of cone snails. Among 29 superfamilies based on conserved signal sequences, T-superfamily conotoxins, which belong to the smallest group, include four different frameworks that contain four cysteines denominated I, V, X and XVI. In this work, the primary structure and the cysteine connectivity of novel conotoxin of <i>Conus bandanus</i> were determined by tandem mass spectrometry using collision-induced dissociation.<h4>Methods</h4>The venom glands of <i>C. bandanus</i> snails were dissected, pooled, and extracted with 0.1% trifluoroacetic acid in three steps and lyophilized. The venom was fractionated and purified in an HPLC system with an analytical reversed-phase C₁₈ coThe journal of venomous animals and toxins including tropical diseasese20190095https://www.ebi.ac.uk/biostudies/studies/S-EPMC7216822biostudies-literatureIsolation and structural identification of a new T1-conotoxin with unique disulfide connectivities derived from <i>Conus bandanus</i>.PMC7216822Bao NVinh PTKNghia NDLecaer JPfalseIsolation and structural identification of a new T1-conotoxin with unique disulfide connectivities derived from <i>Conus bandanus</i>.<h4>Background</h4>Conopeptides are neuropharmacological peptides derived from the venomous salivary glands of cone snails. Among 29 superfamilies based on conserved signal sequences, T-superfamily conotoxins, which belong to the smallest group, include four different frameworks that contain four cysteines denominated I, V, X and XVI. In this work, the primary structure and the cysteine connectivity of novel conotoxin of <i>Conus bandanus</i> were determined by tandem mass spectrometry using collision-induced dissociation.<h4>Methods</h4>The venom glands of <i>C. bandanus</i> snails were dissected, pooled, and extracted with 0.1% trifluoroacetic acid in three steps and lyophilized. The venom was fractionated and purified in an HPLC system with an analytical reversed-phase C₁₈ co2020-01-01T00:00:00Z20202025-04-27T00:22:57.881Z2020-05-23T07:05:34ZS-EPMC72168223242599310.1590/1678-9199-JVATITD-2019-0095