biostudies-literaturevan de Veen WSwiss National Science FoundationSwiss Cancer Research FoundationPromedica StiftungChristine Kühne – Center for Allergy Research and Educationeaaz3559https://www.ebi.ac.uk/biostudies/studies/S-EPMC7220305biostudies-literatureUnknown6(20)B cells contribute to immune responses through the production of immunoglobulins, antigen presentation, and cytokine production. Several B cell subsets with distinct functions and polarized cytokine profiles have been reported. In this study, we used transcriptomics analysis of immortalized B cell clones to identify an IgG4⁺ B cell subset with a unique function. These B cells are characterized by simultaneous expression of proangiogenic cytokines including VEGF, CYR61, ADM, FGF2, PDGFA, and MDK. Consequently, supernatants from these clones efficiently promote endothelial cell tube formation. We identified CD49b and CD73 as surface markers identifying proangiogenic B cells. Circulating CD49b⁺CD73⁺ B cells showed significantly increased frequency in patients Science advancesA novel proangiogenic B cell subset is increased in cancer and chronic inflammation.PMC7220305320030210030_179428320030-159870KFS-4243-08-20171513261574482100301794281406/M & 1412/MPP00P3_157448PMPDP3_151326159870Ochsner UStraumann AIgnatova Dvan Splunter MAkdis MKubo TWirz OFCastro-Giner FWallimann AChang YTFonseca Guevara RJVerschoor DHerrmann MFlatz LTan GRuckert BGuenova EHuntjens Dvan de Veen WSpits HAkdis CAStanic BJansen KFassnacht CGlobinska AfalseA novel proangiogenic B cell subset is increased in cancer and chronic inflammation.B cells contribute to immune responses through the production of immunoglobulins, antigen presentation, and cytokine production. Several B cell subsets with distinct functions and polarized cytokine profiles have been reported. In this study, we used transcriptomics analysis of immortalized B cell clones to identify an IgG4⁺ B cell subset with a unique function. These B cells are characterized by simultaneous expression of proangiogenic cytokines including VEGF, CYR61, ADM, FGF2, PDGFA, and MDK. Consequently, supernatants from these clones efficiently promote endothelial cell tube formation. We identified CD49b and CD73 as surface markers identifying proangiogenic B cells. Circulating CD49b⁺CD73⁺ B cells showed significantly increased frequency in patients 2020-01-01T00:00:00Z2020 May2026-04-17T15:14:40.499Z2020-05-23T07:06:35ZS-EPMC72203053242649710.1126/sciadv.aaz3559