biostudies-literatureChao CMDeutsche Forschungsgemeinschaft, DZL, First Affiliated Hospital of Wenzhou Medical UniversityE859https://www.ebi.ac.uk/biostudies/studies/S-EPMC7226730biostudies-literatureUnknown9(4)<h4>Background</h4>Bronchopulmonary dysplasia (BPD) is a lung disease of preterm born infants, characterized by alveolar simplification. MicroRNA (<i>miR)</i> are known to be involved in many biological and pathological processes in the lung. Although a changed expression has been described for several <i>miR</i> in BPD, a causal role remains to be established.<h4>Results</h4>Our results showed that the expression level of <i>miR-154</i> increases during lung development and decreases postnatally. Further, hyperoxia treatment maintains high levels of <i>miR-154</i> in alveolar type 2 cells (AT2). We hypothesized that the decrease in <i>miR-154</i> expression in AT2 cells is required for normal alveologenesis. To test this hypothesis, we generated a novel transgenic mouse allowing doxycycliCellsFailure to Down-Regulate <i>miR-154</i> Expression in Early Postnatal Mouse Lung Epithelium Suppresses Alveologenesis, with Changes in Tgf-β Signaling Similar to those Induced by Exposure to Hyperoxia.PMC7226730DFG; BE4443/14-1, BE4443/6-1, KFO309 P7 and SFB CRC1213-projects A02 and A04Clinical career grantWu JBarreto GBellusci SSedighi JChong LKolck JMinoo PMoiseenko ARako ZACarraro GWarburton DYoung BMChao CMContreras AZimmermann VWilhelm JfalseFailure to Down-Regulate <i>miR-154</i> Expression in Early Postnatal Mouse Lung Epithelium Suppresses Alveologenesis, with Changes in Tgf-β Signaling Similar to those Induced by Exposure to Hyperoxia.<h4>Background</h4>Bronchopulmonary dysplasia (BPD) is a lung disease of preterm born infants, characterized by alveolar simplification. MicroRNA (<i>miR)</i> are known to be involved in many biological and pathological processes in the lung. Although a changed expression has been described for several <i>miR</i> in BPD, a causal role remains to be established.<h4>Results</h4>Our results showed that the expression level of <i>miR-154</i> increases during lung development and decreases postnatally. Further, hyperoxia treatment maintains high levels of <i>miR-154</i> in alveolar type 2 cells (AT2). We hypothesized that the decrease in <i>miR-154</i> expression in AT2 cells is required for normal alveologenesis. To test this hypothesis, we generated a novel transgenic mouse allowing doxycycli2020-01-01T00:00:00Z2020 Apr2026-04-17T15:24:07.67Z2020-05-23T07:09:43ZS-EPMC72267303225234110.3390/cells9040859