{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Tang H"],"pubmed_abstract":["Staphylococcus aureus relies on quorum sensing to exert virulence to establish and maintain infection. Prior research demonstrated the potent quorum sensing inhibition effects of \"430D-F5\", a refined extract derived from the fruits of Schinus terebinthifolia, a medicinal plant used for the traditional treatment of skin and soft tissue infections. We report the isolation and identification of three compounds from 430D-F5 that reduce virulence and abate dermonecrosis: 3-oxo-olean-12-en-28-oic acid (1), 3-oxotirucalla-7,24Z-dien-26-oic acid (2) and 3?-hydroxytirucalla-7,24 Z-dien-27-oic acid (3). Each compound inhibits all S. aureus accessory gene regulator (agr) alleles (IC<sub>50</sub> 2-70??M). Dose-dependent responses were also observed in agr-regulated reporters for leucocidin A (lukA, IC<sub>50</sub> 0.4-25??M) and glycerol ester hydrolase or lipase (gehB, IC<sub>50</sub> 1.5-25??M). Surprisingly, dose-dependent activity against the nuclease reporter (nuc), which is under the control of the sae two-component system, was also observed (IC<sub>50</sub> 0.4-12.5??M). Compounds 1-3 exhibited little to no effect on the agr-independent mgrA P2 reporter (a constitutive promoter from the mgrA two-component system) and the esxA reporter (under control of mgrA). Compounds 1-3 inhibited ?-toxin production in vitro and reduced dermonecrosis in a murine in vivo model. This is the first report of triterpenoid acids with potent anti-virulence effects against S. aureus."],"journal":["Scientific reports"],"pagination":["8046"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7229044"],"repository":["biostudies-literature"],"pubmed_title":["Triterpenoid acids isolated from Schinus terebinthifolia fruits reduce Staphylococcus aureus virulence and abate dermonecrosis."],"pmcid":["PMC7229044"],"pubmed_authors":["Porras G","Bacsa J","Quave CL","Brown MM","Lyles JT","Horswill AR","Chassagne F","Tang H"],"additional_accession":[]},"is_claimable":false,"name":"Triterpenoid acids isolated from Schinus terebinthifolia fruits reduce Staphylococcus aureus virulence and abate dermonecrosis.","description":"Staphylococcus aureus relies on quorum sensing to exert virulence to establish and maintain infection. Prior research demonstrated the potent quorum sensing inhibition effects of \"430D-F5\", a refined extract derived from the fruits of Schinus terebinthifolia, a medicinal plant used for the traditional treatment of skin and soft tissue infections. We report the isolation and identification of three compounds from 430D-F5 that reduce virulence and abate dermonecrosis: 3-oxo-olean-12-en-28-oic acid (1), 3-oxotirucalla-7,24Z-dien-26-oic acid (2) and 3?-hydroxytirucalla-7,24 Z-dien-27-oic acid (3). Each compound inhibits all S. aureus accessory gene regulator (agr) alleles (IC<sub>50</sub> 2-70??M). Dose-dependent responses were also observed in agr-regulated reporters for leucocidin A (lukA, IC<sub>50</sub> 0.4-25??M) and glycerol ester hydrolase or lipase (gehB, IC<sub>50</sub> 1.5-25??M). Surprisingly, dose-dependent activity against the nuclease reporter (nuc), which is under the control of the sae two-component system, was also observed (IC<sub>50</sub> 0.4-12.5??M). Compounds 1-3 exhibited little to no effect on the agr-independent mgrA P2 reporter (a constitutive promoter from the mgrA two-component system) and the esxA reporter (under control of mgrA). Compounds 1-3 inhibited ?-toxin production in vitro and reduced dermonecrosis in a murine in vivo model. This is the first report of triterpenoid acids with potent anti-virulence effects against S. aureus.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 May","modification":"2021-02-21T07:07:24Z","creation":"2020-05-30T07:03:53Z"},"accession":"S-EPMC7229044","cross_references":{"pubmed":["32415287"],"doi":["10.1038/s41598-020-65080-3"]}}