biostudies-literatureXiao QMedical Science Research Project of Hebei Province of ChinaNatural Science Foundation for Excellent Youth Scholars of Hebei Province of ChinaChinese Academy of Agricultural Sciences161-171https://www.ebi.ac.uk/biostudies/studies/S-EPMC7231830biostudies-literatureUnknown58(2)The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.The Korean journal of parasitologyChanges in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis.PMC7231830SKLVEB2018KFKT00720190036C2017205135Yang XWang MTang JLiu JHu YWang XWang HXue XLi MZhao YXiao QfalseChanges in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis.The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.2020-01-01T00:00:00Z2020 Apr2024-02-15T19:44:17.597Z2020-06-02T07:11:40ZS-EPMC72318303241838510.3347/kjp.2020.58.2.161