{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Malysheva SV"],"funding":["Belgian National Reference Laboratory for Mycotoxins, Plant toxins and Marine Biotoxins"],"pagination":["E243"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7232191"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(4)"],"pubmed_abstract":["Cardiac glycosides (CGs) are naturally occurring plant secondary metabolites that can be toxic to humans and animals. The aim of this work was to develop a targeted analytical method utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for quantification of these plant toxins in a herbal-based food and human urine. The method included oleandrin, digoxin, digitoxin, convallatoxin, and ouabain. Samples of culinary herbs were extracted with acetonitrile and cleaned using Oasis<sup>®</sup> MAX solid-phase extraction (SPE), while samples of urine were diluted with acidified water and purified on Oasis<sup>®</sup> HLB SPE cartridges. Limits of quantification were in the range of 1.5-15 ng/g for herbs and 0.025-1 ng/mL for urine. The mean recovery of the method complied with the ac"],"journal":["Toxins"],"pubmed_title":["Development and Validation of a UHPLC-ESI-MS/MS Method for Quantification of Oleandrin and Other Cardiac Glycosides and Evaluation of Their Levels in Herbs and Spices from the Belgian Market."],"pmcid":["PMC7232191"],"funding_grant_id":["FAVV-NRL-GGO-TOX-2017, lot 2"],"pubmed_authors":["Malysheva SV","Mulder PPJ","Masquelier J"],"additional_accession":[]},"is_claimable":false,"name":"Development and Validation of a UHPLC-ESI-MS/MS Method for Quantification of Oleandrin and Other Cardiac Glycosides and Evaluation of Their Levels in Herbs and Spices from the Belgian Market.","description":"Cardiac glycosides (CGs) are naturally occurring plant secondary metabolites that can be toxic to humans and animals. The aim of this work was to develop a targeted analytical method utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for quantification of these plant toxins in a herbal-based food and human urine. The method included oleandrin, digoxin, digitoxin, convallatoxin, and ouabain. Samples of culinary herbs were extracted with acetonitrile and cleaned using Oasis<sup>®</sup> MAX solid-phase extraction (SPE), while samples of urine were diluted with acidified water and purified on Oasis<sup>®</sup> HLB SPE cartridges. Limits of quantification were in the range of 1.5-15 ng/g for herbs and 0.025-1 ng/mL for urine. The mean recovery of the method complied with the ac","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-07-15T22:08:18.23Z","creation":"2020-05-26T07:11:18Z"},"accession":"S-EPMC7232191","cross_references":{"pubmed":["32283845"],"doi":["10.3390/toxins12040243"]}}