biostudies-literature00550Gopinath TAmerican Heart AssociationNHLBI NIH HHSNIH HHSNIGMS NIH HHS267-285https://www.ebi.ac.uk/biostudies/studies/S-EPMC7236978biostudies-literatureUnknown74(4-5)Ultrafast magic angle spinning (MAS) technology and ¹H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional ¹H- or ¹³C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of ¹H and ¹³C nuclei. We also expand this approach to record ¹H-, ¹³C-, and ¹⁵N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.Journal of biomolecular NMRMulti-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning.PMC72369781S10OD021536GM 64742R01 GM064742S10 OD021536R01 HL144130HL 14413019POST34420009Gopinath TVeglia GWeber DK55falseMulti-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning.Ultrafast magic angle spinning (MAS) technology and ¹H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional ¹H- or ¹³C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of ¹H and ¹³C nuclei. We also expand this approach to record ¹H-, ¹³C-, and ¹⁵N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.2020-01-01T00:00:00Z2020 May2022-02-09T18:08:56.963Z2022-02-09T18:08:56.963ZS-EPMC72369783233319310.1007/s10858-020-00316-y