{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tattikota SG"],"funding":["NCRR NIH HHS","Howard Hughes Medical Institute","Harvard Medical School","Samsung Science and Technology Foundation"],"pagination":["e54818"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7237219"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["<i>Drosophila</i> blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand <i>branchless</i> and receptor <i>breathless</i>, respectively. We demonstrate that these FGF compon"],"journal":["eLife"],"pubmed_title":["A single-cell survey of <i>Drosophila</i> blood."],"pmcid":["PMC7237219"],"funding_grant_id":["UL1 RR025758","SSTF-BA1701-15","S10 RR028832"],"pubmed_authors":["Barrera V","Steinbaugh MJ","Yoon SH","Hu Y","Perrimon N","Li F","Ho Sui S","Dervis F","Tattikota SG","Cho B","Shim J","Liu Y","Nam JW","Hung RJ","Comjean A"],"additional_accession":[]},"is_claimable":false,"name":"A single-cell survey of <i>Drosophila</i> blood.","description":"<i>Drosophila</i> blood cells, called hemocytes, are classified into plasmatocytes, crystal cells, and lamellocytes based on the expression of a few marker genes and cell morphologies, which are inadequate to classify the complete hemocyte repertoire. Here, we used single-cell RNA sequencing (scRNA-seq) to map hemocytes across different inflammatory conditions in larvae. We resolved plasmatocytes into different states based on the expression of genes involved in cell cycle, antimicrobial response, and metabolism together with the identification of intermediate states. Further, we discovered rare subsets within crystal cells and lamellocytes that express fibroblast growth factor (FGF) ligand <i>branchless</i> and receptor <i>breathless</i>, respectively. We demonstrate that these FGF compon","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 May","modification":"2026-05-04T13:27:45.082Z","creation":"2020-05-24T07:13:12Z"},"accession":"S-EPMC7237219","cross_references":{"pubmed":["32396065"],"doi":["10.7554/eLife.54818"]}}