biostudies-literatureUnknown9(4)Durrani HIn kinetoplastids, the first seven steps of glycolysis are compartmentalized into a glycosome along with parts of other metabolic pathways. This organelle shares a common ancestor with the better-understood eukaryotic peroxisome. Much of our understanding of the emergence, evolution, and maintenance of glycosomes is limited to explorations of the dixenous parasites, including the enzymatic contents of the organelle. Our objective was to determine the extent that we could leverage existing studies in model kinetoplastids to determine the composition of glycosomes in species lacking evidence of experimental localization. These include diverse monoxenous species and dixenous species with very different hosts. For many of these, genome or transcriptome sequences are available. Our approach iniPathogens (Basel, Switzerland)E281https://www.ebi.ac.uk/biostudies/studies/S-EPMC7237986biostudies-literatureA Global Analysis of Enzyme Compartmentalization to Glycosomes.PMC7237986Durrani HZimmer SLHampton MRumbley JNfalseA Global Analysis of Enzyme Compartmentalization to Glycosomes.In kinetoplastids, the first seven steps of glycolysis are compartmentalized into a glycosome along with parts of other metabolic pathways. This organelle shares a common ancestor with the better-understood eukaryotic peroxisome. Much of our understanding of the emergence, evolution, and maintenance of glycosomes is limited to explorations of the dixenous parasites, including the enzymatic contents of the organelle. Our objective was to determine the extent that we could leverage existing studies in model kinetoplastids to determine the composition of glycosomes in species lacking evidence of experimental localization. These include diverse monoxenous species and dixenous species with very different hosts. For many of these, genome or transcriptome sequences are available. Our approach ini2020-01-01T00:00:00Z2020 Apr2026-04-07T19:55:58.374Z2020-06-01T07:05:07ZS-EPMC72379863229058810.3390/pathogens9040281