{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stenzel I"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["E452"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7238183"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(4)"],"pubmed_abstract":["Polar tip growth of pollen tubes is regulated by the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), which localizes in a well-defined region of the subapical plasma membrane. How the PtdIns(4,5)P2 region is maintained is currently unclear. In principle, the formation of PtdIns(4,5)P2 by PI4P 5-kinases can be counteracted by phospholipase C (PLC), which hydrolyzes PtdIns(4,5)P2. Here, we show that fluorescence-tagged tobacco NtPLC3 displays a subapical plasma membrane distribution which frames that of fluorescence-tagged PI4P 5-kinases, suggesting that NtPLC3 may modulate PtdIns(4,5)P2-mediated processes in pollen tubes. The expression of a dominant negative NtPLC3 variant resulted in pollen tube tip swelling, consistent with a delimiting effect on PtdIns(4,5)P"],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["Coordinated Localization and Antagonistic Function of NtPLC3 and PI4P 5-Kinases in the Subapical Plasma Membrane of Tobacco Pollen Tubes."],"pmcid":["PMC7238183"],"funding_grant_id":["INST271/371-1 FUGG","He3424/1","He3424/6-1"],"pubmed_authors":["Ischebeck T","Riechmann M","Heilmann I","Fratini M","Krishnamoorthy P","Stenzel I","Vu-Becker LH"],"additional_accession":[]},"is_claimable":false,"name":"Coordinated Localization and Antagonistic Function of NtPLC3 and PI4P 5-Kinases in the Subapical Plasma Membrane of Tobacco Pollen Tubes.","description":"Polar tip growth of pollen tubes is regulated by the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), which localizes in a well-defined region of the subapical plasma membrane. How the PtdIns(4,5)P2 region is maintained is currently unclear. In principle, the formation of PtdIns(4,5)P2 by PI4P 5-kinases can be counteracted by phospholipase C (PLC), which hydrolyzes PtdIns(4,5)P2. Here, we show that fluorescence-tagged tobacco NtPLC3 displays a subapical plasma membrane distribution which frames that of fluorescence-tagged PI4P 5-kinases, suggesting that NtPLC3 may modulate PtdIns(4,5)P2-mediated processes in pollen tubes. The expression of a dominant negative NtPLC3 variant resulted in pollen tube tip swelling, consistent with a delimiting effect on PtdIns(4,5)P","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2025-04-04T11:44:53.682Z","creation":"2020-06-01T07:07:21Z"},"accession":"S-EPMC7238183","cross_references":{"pubmed":["32260253"],"doi":["10.3390/plants9040452"]}}