biostudies-literatureGomez EANPCyTCONICETInstituto Nacional de Tecnología Agropecuaria95https://www.ebi.ac.uk/biostudies/studies/S-EPMC7239984biostudies-literatureUnknown10(1)Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We develoAMB ExpressDevelopment of diagnostic tools for IBDV detection using plants as bioreactors.PMC7239984PICT 2011-1023PNBIO 1131032PIP 11220110100064PICT 2016-313Cassani MFLucero MSChimeno Zoth SGomez EBerinstein AParreno VfalseDevelopment of diagnostic tools for IBDV detection using plants as bioreactors.Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We develo2020-01-01T00:00:00Z2020 May2025-04-26T00:20:31.02Z2020-05-31T07:06:22ZS-EPMC72399843243605710.1186/s13568-020-01029-z