biostudies-literatureUnknown27(6)Xu CHuman telomerase RNA component hTERC comprises multiple motifs that contribute to hTERC biogenesis, holoenzyme activity, and enzyme recruitment to telomeres. hTERC contains several guanine tracts (G-tracts) at its 5'-end, but its associated proteins and potential roles in telomerase function are still poorly understood. The heterogeneous nuclear ribonucleoproteins F, H1, and H2 (hnRNP F/H) are splicing factors that preferentially bind to poly(G)-rich sequences RNA. Here, we demonstrate that hnRNP F/H associate with both hTERC and telomerase holoenzyme to regulate telomerase activity. We reveal hnRNP F/H bind to the 5'-end region of hTERC in vitro and in vivo, and identify the first three G-tracts of hTERC and qRRM1 domain of hnRNP F/H are required for their interaction. Furthermore, hnRNP Cell death and differentiation1998-2013https://www.ebi.ac.uk/biostudies/studies/S-EPMC7244589biostudies-literatureHnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation.PMC7244589Li GHan LChen JLiang YXing XXu CZhang NXie NSun ZTong TJia SSu YLiu DfalseHnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation.Human telomerase RNA component hTERC comprises multiple motifs that contribute to hTERC biogenesis, holoenzyme activity, and enzyme recruitment to telomeres. hTERC contains several guanine tracts (G-tracts) at its 5'-end, but its associated proteins and potential roles in telomerase function are still poorly understood. The heterogeneous nuclear ribonucleoproteins F, H1, and H2 (hnRNP F/H) are splicing factors that preferentially bind to poly(G)-rich sequences RNA. Here, we demonstrate that hnRNP F/H associate with both hTERC and telomerase holoenzyme to regulate telomerase activity. We reveal hnRNP F/H bind to the 5'-end region of hTERC in vitro and in vivo, and identify the first three G-tracts of hTERC and qRRM1 domain of hnRNP F/H are required for their interaction. Furthermore, hnRNP 2020-01-01T00:00:00Z2020 Jun2025-04-04T23:40:02.419Z2020-06-07T07:13:07ZS-EPMC72445893186306910.1038/s41418-019-0483-6