biostudies-literatureAdam KNational Institutes of HealthE3319https://www.ebi.ac.uk/biostudies/studies/S-EPMC7247550biostudies-literatureUnknown21(9)Neuroblastoma is the most common extracranial solid tumor in childhood. Gain of chromosome 17q material is found in >60% of neuroblastoma tumors and is associated with poor patient prognosis. The NME1 gene is located in the 17q21.3 region, and high NME1 expression is correlated with poor neuroblastoma patient outcomes. However, the functional roles and signaling activity of NME1 in neuroblastoma cells and tumors are unknown. NME1 and NME2 have been shown to possess histidine (His) kinase activity. Using anti-1- and 3-pHis specific monoclonal antibodies and polyclonal anti-pH118 NME1/2 antibodies, we demonstrated the presence of pH118-NME1/2 and multiple additional pHis-containing proteins in all tested neuroblastoma cell lines and in xenograft neuroblastoma tumors, supporting the presence International journal of molecular sciencesThe Potential Functional Roles of NME1 Histidine Kinase Activity in Neuroblastoma Pathogenesis.PMC72475501R01CA1945841R35CA242443Zage PEHunter TAdam KLesperance JfalseThe Potential Functional Roles of NME1 Histidine Kinase Activity in Neuroblastoma Pathogenesis.Neuroblastoma is the most common extracranial solid tumor in childhood. Gain of chromosome 17q material is found in >60% of neuroblastoma tumors and is associated with poor patient prognosis. The NME1 gene is located in the 17q21.3 region, and high NME1 expression is correlated with poor neuroblastoma patient outcomes. However, the functional roles and signaling activity of NME1 in neuroblastoma cells and tumors are unknown. NME1 and NME2 have been shown to possess histidine (His) kinase activity. Using anti-1- and 3-pHis specific monoclonal antibodies and polyclonal anti-pH118 NME1/2 antibodies, we demonstrated the presence of pH118-NME1/2 and multiple additional pHis-containing proteins in all tested neuroblastoma cell lines and in xenograft neuroblastoma tumors, supporting the presence 2020-01-01T00:00:00Z2020 May2025-05-29T19:17:32.439Z2025-05-29T19:17:32.439ZS-EPMC72475503239288910.3390/ijms21093319