{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wei L"],"funding":["ShiHeZi University"],"pagination":["9549765"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7254085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2020"],"pubmed_abstract":["Zinc finger protein X-linked (<i>Zfx</i>) was regarded to be a sex determination factor and plays a critical role in spermatogenesis. RNAi is an effective method of silencing <i>Zfx</i> mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of red deer (<i>Cervus elaphus</i>). The objective of this study was first to explore an efficient method to alter the red deer offspring sex-ratio by silencing the gene <i>Zfx</i> during spermatogenesis. Three recombinant expression vectors pLL3.7/A, pLL3.7/B, and pLL3.7/C were constructed to interrupt the <i>Zfx</i> gene. The results showed that the expression of <i>Zfx</i> mRNA was significantly silenced by pLL3.7/A (<i>P</i> < 0.01), compared with the control group. The group inject"],"journal":["BioMed research international"],"pubmed_title":["The Use of RNAi Technology to Interfere with <i>Zfx</i> Gene Increases the Male Rates of Red Deer (<i>Cervus elaphus</i>) Offspring."],"pmcid":["PMC7254085"],"funding_grant_id":["RCZK2018C13"],"pubmed_authors":["Zeng B","Xi J","Li Y","Zhang Y","Li F","Jia B","Jiang S","Zhang S","Shen H","Wei L","Wang K","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"The Use of RNAi Technology to Interfere with <i>Zfx</i> Gene Increases the Male Rates of Red Deer (<i>Cervus elaphus</i>) Offspring.","description":"Zinc finger protein X-linked (<i>Zfx</i>) was regarded to be a sex determination factor and plays a critical role in spermatogenesis. RNAi is an effective method of silencing <i>Zfx</i> mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of red deer (<i>Cervus elaphus</i>). The objective of this study was first to explore an efficient method to alter the red deer offspring sex-ratio by silencing the gene <i>Zfx</i> during spermatogenesis. Three recombinant expression vectors pLL3.7/A, pLL3.7/B, and pLL3.7/C were constructed to interrupt the <i>Zfx</i> gene. The results showed that the expression of <i>Zfx</i> mRNA was significantly silenced by pLL3.7/A (<i>P</i> < 0.01), compared with the control group. The group inject","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-04T08:04:15.584Z","creation":"2020-06-10T07:08:37Z"},"accession":"S-EPMC7254085","cross_references":{"pubmed":["32509876"],"doi":["10.1155/2020/9549765"]}}