biostudies-literatureWei LShiHeZi University9549765https://www.ebi.ac.uk/biostudies/studies/S-EPMC7254085biostudies-literatureUnknown2020Zinc finger protein X-linked (<i>Zfx</i>) was regarded to be a sex determination factor and plays a critical role in spermatogenesis. RNAi is an effective method of silencing <i>Zfx</i> mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of red deer (<i>Cervus elaphus</i>). The objective of this study was first to explore an efficient method to alter the red deer offspring sex-ratio by silencing the gene <i>Zfx</i> during spermatogenesis. Three recombinant expression vectors pLL3.7/A, pLL3.7/B, and pLL3.7/C were constructed to interrupt the <i>Zfx</i> gene. The results showed that the expression of <i>Zfx</i> mRNA was significantly silenced by pLL3.7/A (<i>P</i> < 0.01), compared with the control group. The group injectBioMed research internationalThe Use of RNAi Technology to Interfere with <i>Zfx</i> Gene Increases the Male Rates of Red Deer (<i>Cervus elaphus</i>) Offspring.PMC7254085RCZK2018C13Zeng BXi JLi YZhang YLi FJia BJiang SZhang SShen HWei LWang KZhao XfalseThe Use of RNAi Technology to Interfere with <i>Zfx</i> Gene Increases the Male Rates of Red Deer (<i>Cervus elaphus</i>) Offspring.Zinc finger protein X-linked (<i>Zfx</i>) was regarded to be a sex determination factor and plays a critical role in spermatogenesis. RNAi is an effective method of silencing <i>Zfx</i> mRNA expression. However, there has been little research on the use of RNAi technology to control the sex of the offspring of red deer (<i>Cervus elaphus</i>). The objective of this study was first to explore an efficient method to alter the red deer offspring sex-ratio by silencing the gene <i>Zfx</i> during spermatogenesis. Three recombinant expression vectors pLL3.7/A, pLL3.7/B, and pLL3.7/C were constructed to interrupt the <i>Zfx</i> gene. The results showed that the expression of <i>Zfx</i> mRNA was significantly silenced by pLL3.7/A (<i>P</i> < 0.01), compared with the control group. The group inject2020-01-01T00:00:00Z20202025-04-04T08:04:15.584Z2020-06-10T07:08:37ZS-EPMC72540853250987610.1155/2020/9549765