biostudies-literatureChen HState Administration of Traditional Chinese Medicine of the People&apos;s Republic of China1706837https://www.ebi.ac.uk/biostudies/studies/S-EPMC7256709biostudies-literatureUnknown2020The inflammatory proliferation of fibroblast-like synoviocytes (FLSs) and functional imbalances in T lymphocytes play critical roles in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of Huayu Tongbi Fang (HYTB, a traditional herbal formula) in RA treatment has been validated. In this study, we aimed to explore the regulatory mechanisms of HYTB on the proliferation and differentiation of T lymphocytes, and the inhibitory effect of HYTB on inflammatory proliferation of FLSs. The RCS-364 (Rat FLSs) cells were cocultured with rat splenic lymphocytes that were induced by interleukin-1<i>β</i> in Transwell chambers. After freeze-dried HYTB powder treatment, the percentage of T-cell subset and apoptosis rates of FLSs were measured using flow cytometry. Furthermore, protein eEvidence-based complementary and alternative medicine : eCAMChinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1<i>β</i> by Regulating Proliferation and Differentiation of T Lymphocytes.PMC7256709JDZX20151912019-JYB-XS-177Li XLiu JChai LLi JWang CZhang HHuandike MChen HHuang QZhou JfalseChinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1<i>β</i> by Regulating Proliferation and Differentiation of T Lymphocytes.The inflammatory proliferation of fibroblast-like synoviocytes (FLSs) and functional imbalances in T lymphocytes play critical roles in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of Huayu Tongbi Fang (HYTB, a traditional herbal formula) in RA treatment has been validated. In this study, we aimed to explore the regulatory mechanisms of HYTB on the proliferation and differentiation of T lymphocytes, and the inhibitory effect of HYTB on inflammatory proliferation of FLSs. The RCS-364 (Rat FLSs) cells were cocultured with rat splenic lymphocytes that were induced by interleukin-1<i>β</i> in Transwell chambers. After freeze-dried HYTB powder treatment, the percentage of T-cell subset and apoptosis rates of FLSs were measured using flow cytometry. Furthermore, protein e2020-01-01T00:00:00Z20202025-04-19T19:08:06.116Z2020-06-26T07:02:02ZS-EPMC72567093256584710.1155/2020/1706837