{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chikazawa M"],"funding":["MEXT | Japan Society for the Promotion of Science","Japan Agency for Medical Research and Development"],"pagination":["7697-7709"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7261792"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["295(22)"],"pubmed_abstract":["Lysine <i>N</i>-pyrrolation converts lysine residues to <i>N</i><sup>ϵ</sup>-pyrrole-l-lysine (pyrK) in a covalent modification reaction that significantly affects the chemical properties of proteins, causing them to mimic DNA. pyrK in proteins has been detected <i>in vivo</i>, indicating that pyrrolation occurs as an endogenous reaction. However, the source of pyrK remains unknown. In this study, on the basis of our observation <i>in vitro</i> that pyrK is present in oxidized low-density lipoprotein and in modified proteins with oxidized polyunsaturated fatty acids, we used LC-electrospray ionization-MS/MS coupled with a stable isotope dilution method to perform activity-guided separation of active molecules in oxidized lipids and identified glycolaldehyde (GA) as a pyrK source. The resul"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Glycolaldehyde is an endogenous source of lysine <i>N</i>-pyrrolation."],"pmcid":["PMC7261792"],"funding_grant_id":["26111011","18gm0910013h0002","17H06170"],"pubmed_authors":["Chikazawa M","Negishi L","Yoshitake J","Shibata T","Iwata S","Lim SY","Uchida K"],"additional_accession":[]},"is_claimable":false,"name":"Glycolaldehyde is an endogenous source of lysine <i>N</i>-pyrrolation.","description":"Lysine <i>N</i>-pyrrolation converts lysine residues to <i>N</i><sup>ϵ</sup>-pyrrole-l-lysine (pyrK) in a covalent modification reaction that significantly affects the chemical properties of proteins, causing them to mimic DNA. pyrK in proteins has been detected <i>in vivo</i>, indicating that pyrrolation occurs as an endogenous reaction. However, the source of pyrK remains unknown. In this study, on the basis of our observation <i>in vitro</i> that pyrK is present in oxidized low-density lipoprotein and in modified proteins with oxidized polyunsaturated fatty acids, we used LC-electrospray ionization-MS/MS coupled with a stable isotope dilution method to perform activity-guided separation of active molecules in oxidized lipids and identified glycolaldehyde (GA) as a pyrK source. The resul","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 May","modification":"2025-05-18T12:04:34.499Z","creation":"2025-05-18T12:04:34.499Z"},"accession":"S-EPMC7261792","cross_references":{"pubmed":["32332094"],"doi":["10.1074/jbc.RA120.013179","10.1074/jbc.ra120.013179"]}}