{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cao MX"],"funding":["National Key R&D Program of China","National Natural Science Foundation of China"],"pagination":["102"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7268480"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(1)"],"pubmed_abstract":["Background
Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.Methods
miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, Immunohistochemi"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression."],"pmcid":["PMC7268480"],"funding_grant_id":["81672672, 81972542, 81902779 and 21838002","2019YFA09005700"],"pubmed_authors":["Jiang J","Tang YL","Cao MX","Wu JS","Wu JB","Tang YJ","Qiao XW","Huang MC","Liang XH","Zhang WL","Wang K","Yu XH"],"additional_accession":[]},"is_claimable":false,"name":"Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.","description":"Background
Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.Methods
miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, Immunohistochemi","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2025-04-04T13:59:38.879Z","creation":"2020-06-13T07:11:09Z"},"accession":"S-EPMC7268480","cross_references":{"pubmed":["32493454"],"doi":["10.1186/s13046-020-01602-1"]}}