biostudies-literatureCao MXNational Key R&amp;D Program of ChinaNational Natural Science Foundation of China102https://www.ebi.ac.uk/biostudies/studies/S-EPMC7268480biostudies-literatureUnknown39(1)<h4>Background</h4>Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.<h4>Methods</h4>miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, ImmunohistochemiJournal of experimental & clinical cancer research : CRInterplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.PMC726848081672672, 81972542, 81902779 and 218380022019YFA09005700Jiang JTang YLCao MXWu JSWu JBTang YJQiao XWHuang MCLiang XHZhang WLWang KYu XHfalseInterplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.<h4>Background</h4>Human papillomavirus (HPV)-positive oral squamous cell carcinoma (OSCC) is increasing worldwide with typically higher grade and stage, while better prognosis. microRNAs (miRNAs) has been shown to play a critical role in cancer, however, their role in HPV-positive OSCC progression remains unclear.<h4>Methods</h4>miRNA microarray was performed to identify differentially expressed miRNAs. qRT-PCR and FISH were performed to determine the relative expression of miR-550a-3-5p. CCK-8, Flow cytometry, Wound healing, Cell invasion assays and xenograft experiments were conducted to analyze the biological roles of miR-550a-3-5p. Tumor-associated macrophages (TAMs) generation, co-culturing of cancer cells with TAMs, Western blot, Dual-luciferase reporter gene assay, Immunohistochemi2020-01-01T00:00:00Z2020 Jun2025-04-04T13:59:38.879Z2020-06-13T07:11:09ZS-EPMC72684803249345410.1186/s13046-020-01602-1