{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wirka RC"],"funding":["NIDDK NIH HHS","NCRR NIH HHS","NHLBI NIH HHS","NIH HHS"],"pagination":["1280-1289"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7274198"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(8)"],"pubmed_abstract":["In response to various stimuli, vascular smooth muscle cells (SMCs) can de-differentiate, proliferate and migrate in a process known as phenotypic modulation. However, the phenotype of modulated SMCs in vivo during atherosclerosis and the influence of this process on coronary artery disease (CAD) risk have not been clearly established. Using single-cell RNA sequencing, we comprehensively characterized the transcriptomic phenotype of modulated SMCs in vivo in atherosclerotic lesions of both mouse and human arteries and found that these cells transform into unique fibroblast-like cells, termed 'fibromyocytes', rather than into a classical macrophage phenotype. SMC-specific knockout of TCF21-a causal CAD gene-markedly inhibited SMC phenotypic modulation in mice, leading to the presence of few"],"journal":["Nature medicine"],"pubmed_title":["Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis."],"pmcid":["PMC7274198"],"funding_grant_id":["R01 HL109512","P30 DK116074","S10 RR025518","R01 HL134817","K08 HL133375","R21 HL120757","R01 HL148239","R33 HL120757","R01 HL141371","R01 HL144067","R01 HL139478","R01 DK107437","S10 OD018220","R01 HL145708","R00 HL125912","R01 HL103635","F32 HL129670"],"pubmed_authors":["Miller CL","Koyano TK","Quertermous T","Wu JC","Chang R","Montgomery SB","Nguyen T","Alamprese M","Nagao M","Paik DT","Coller J","Kundu R","Wagh D","Wirka RC","Fong R","Zhu K","Pjanic M","Liu B","Kim JB","Woo YJ","Tallquist MD"],"additional_accession":[]},"is_claimable":false,"name":"Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis.","description":"In response to various stimuli, vascular smooth muscle cells (SMCs) can de-differentiate, proliferate and migrate in a process known as phenotypic modulation. However, the phenotype of modulated SMCs in vivo during atherosclerosis and the influence of this process on coronary artery disease (CAD) risk have not been clearly established. Using single-cell RNA sequencing, we comprehensively characterized the transcriptomic phenotype of modulated SMCs in vivo in atherosclerotic lesions of both mouse and human arteries and found that these cells transform into unique fibroblast-like cells, termed 'fibromyocytes', rather than into a classical macrophage phenotype. SMC-specific knockout of TCF21-a causal CAD gene-markedly inhibited SMC phenotypic modulation in mice, leading to the presence of few","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Aug","modification":"2026-04-08T00:51:48.497Z","creation":"2020-06-09T07:14:43Z"},"accession":"S-EPMC7274198","cross_references":{"pubmed":["31359001"],"doi":["10.1038/s41591-019-0512-5"]}}