biostudies-literatureUnknown8Berchem TService Public de Wallonie<i>Cannabis sp</i>. and <i>Euphorbia sp</i>. are potential candidates as indoor culture for the extraction of their high value-added metabolites for pharmaceutical applications. Both residual lignocellulosic materials recovered after extraction are studied in the present article as single or mixed feedstocks for a closed-loop bioprocesses cascade. An alkaline process (NaOH 3%, 30 min 160°C) is performed to separate the studied biomasses into their main components: lignin and cellulose. Results highlight the advantages of the multi-feedstocks approach over the single biomass in term of lignin yield and purity. Since the structural characteristics of lignin affect the potential applications, a particular attention is drawn on the comprehension of lignin structure alteration and the possible Frontiers in chemistry479https://www.ebi.ac.uk/biostudies/studies/S-EPMC7292014biostudies-literatureSingle and Mixed Feedstocks Biorefining: Comparison of Primary Metabolites Recovery and Lignin Recombination During an Alkaline Process.PMC7292014Richel ASchmetz QLepage TBerchem TfalseSingle and Mixed Feedstocks Biorefining: Comparison of Primary Metabolites Recovery and Lignin Recombination During an Alkaline Process.<i>Cannabis sp</i>. and <i>Euphorbia sp</i>. are potential candidates as indoor culture for the extraction of their high value-added metabolites for pharmaceutical applications. Both residual lignocellulosic materials recovered after extraction are studied in the present article as single or mixed feedstocks for a closed-loop bioprocesses cascade. An alkaline process (NaOH 3%, 30 min 160°C) is performed to separate the studied biomasses into their main components: lignin and cellulose. Results highlight the advantages of the multi-feedstocks approach over the single biomass in term of lignin yield and purity. Since the structural characteristics of lignin affect the potential applications, a particular attention is drawn on the comprehension of lignin structure alteration and the possible 2020-01-01T00:00:00Z20202025-06-01T02:55:23.422Z2025-06-01T02:55:23.422ZS-EPMC72920143258264410.3389/fchem.2020.00479