biostudies-literatureUnknown11Kannan NNorges ForskningsrådHelse Midt-Norge<i>Mycobacterium avium</i> (Mav) complex is increasingly reported to cause non-tuberculous infections in individuals with a compromised immune system. Treatment is complicated and no vaccines are available. Previous studies have shown some potential of using genetically modified <i>Mycobacterium smegmatis</i> (Msm) as a vaccine vector to tuberculosis since it is non-pathogenic and thus would be tolerated by immunocompromised individuals. In this study, we used a mutant strain of Msm disrupted in EspG₃, a component of the ESX-3 secretion system. Infection of macrophages and dendritic cells with Msm Δ<i>espG</i> ₃ showed increased antigen presentation compared to cells infected with wild-type Msm. Vaccination of mice with Msm Δ<i>espG</i> ₃, expressing the MaFrontiers in immunology1116https://www.ebi.ac.uk/biostudies/studies/S-EPMC7296097biostudies-literature<i>Mycobacterium smegmatis</i> Vaccine Vector Elicits CD4+ Th17 and CD8+ Tc17 T Cells With Therapeutic Potential to Infections With <i>Mycobacterium avium</i>.PMC7296097Haug MFlo THKannan NSteigedal Mfalse<i>Mycobacterium smegmatis</i> Vaccine Vector Elicits CD4+ Th17 and CD8+ Tc17 T Cells With Therapeutic Potential to Infections With <i>Mycobacterium avium</i>.<i>Mycobacterium avium</i> (Mav) complex is increasingly reported to cause non-tuberculous infections in individuals with a compromised immune system. Treatment is complicated and no vaccines are available. Previous studies have shown some potential of using genetically modified <i>Mycobacterium smegmatis</i> (Msm) as a vaccine vector to tuberculosis since it is non-pathogenic and thus would be tolerated by immunocompromised individuals. In this study, we used a mutant strain of Msm disrupted in EspG₃, a component of the ESX-3 secretion system. Infection of macrophages and dendritic cells with Msm Δ<i>espG</i> ₃ showed increased antigen presentation compared to cells infected with wild-type Msm. Vaccination of mice with Msm Δ<i>espG</i> ₃, expressing the Ma2020-01-01T00:00:00Z20202025-04-19T21:23:52.581Z2020-07-01T07:08:32ZS-EPMC72960973258219610.3389/fimmu.2020.01116