biostudies-literatureBoyapati RKMedical Research Society UKMedical Research CouncilWellcome TrustCrohn's and Colitis UKMRC2113-2122https://www.ebi.ac.uk/biostudies/studies/S-EPMC7301773biostudies-literatureUnknown24(10)<h4>Background</h4>Due to common evolutionary origins, mitochondrial DNA (mtDNA) shares many similarities with immunogenic bacterial DNA. MtDNA is recognized as a pro-inflammatory damage-associated molecular pattern (DAMP) with a pathogenic role in several inflammatory diseases. We hypothesised that mtDNA is released during active disease, serving as a key pro-inflammatory factor in inflammatory bowel disease (IBD).<h4>Methods</h4>Between 2014 and 2015, we collected plasma separated within 2 hours of sampling from 97 prospectively recruited IBD patients (67 ulcerative colitis [UC] and 30 Crohn's disease [CD]) and 40 non-IBD controls. We measured circulating mtDNA using quantitative polymerase chain reaction (amplifying mitochondria COXIII/ND2 genes) and also in mouse colitis induced by dexInflammatory bowel diseasesMitochondrial DNA Is a Pro-Inflammatory Damage-Associated Molecular Pattern Released During Active IBD.PMC7301773M16-1WT096497Vac-982-2016G0701898Kalla RSatsangi JTamborska ALoane JBoyapati RKVentham NTGray MRossi AGDorward DADoherty MKWhitfield PDHo GTfalseMitochondrial DNA Is a Pro-Inflammatory Damage-Associated Molecular Pattern Released During Active IBD.<h4>Background</h4>Due to common evolutionary origins, mitochondrial DNA (mtDNA) shares many similarities with immunogenic bacterial DNA. MtDNA is recognized as a pro-inflammatory damage-associated molecular pattern (DAMP) with a pathogenic role in several inflammatory diseases. We hypothesised that mtDNA is released during active disease, serving as a key pro-inflammatory factor in inflammatory bowel disease (IBD).<h4>Methods</h4>Between 2014 and 2015, we collected plasma separated within 2 hours of sampling from 97 prospectively recruited IBD patients (67 ulcerative colitis [UC] and 30 Crohn's disease [CD]) and 40 non-IBD controls. We measured circulating mtDNA using quantitative polymerase chain reaction (amplifying mitochondria COXIII/ND2 genes) and also in mouse colitis induced by dex2018-01-01T00:00:00Z2018 Sep2025-05-29T21:04:01.678Z2025-05-29T21:04:01.678ZS-EPMC73017732971825510.1093/ibd/izy095