biostudies-literatureDragunas GNHLBI NIH HHSFundação de Amparo à Pesquisa do Estado de São PauloNational Institutes of Health7703-7717https://www.ebi.ac.uk/biostudies/studies/S-EPMC7302963biostudies-literatureUnknown34(6)Parasympathetic neurons in the airways control bronchomotor tone. Increased activity of cholinergic neurons are mediators of airway hyperresponsiveness (AHR) in asthma, however, mechanisms are not elucidated. We describe remodeling of the cholinergic neuronal network in asthmatic airways driven by brain-derived neurotrophic factor (BDNF) and Tropomyosin receptor kinase B (TrkB). Human bronchial biopsies were stained for cholinergic marker vesicular acetylcholine transporter (VAChT). Human lung gene expression and single nucleotide polymorphisms (SNP) in neuroplasticity-related genes were compared between asthma and healthy patients. Wild-type (WT) and mutated TrkB knock-in mice (Ntrk2tm1Ddg/J) with impaired BDNF signaling were chronically exposed to ovalbumin (OVA). Neuronal VAChT stainingFASEB journal : official publication of the Federation of American Societies for Experimental BiologyCholinergic neuroplasticity in asthma driven by TrkB signaling.PMC7302963R01 HL0880292018/18762‐8NIHR01HL088029Ditz BBos STNijboer SVonk JMMunhoz CDvan Asselt JVohlidalova EKistemaker LEMVermeulen CJPrakash YSDragunas GGosens RWoest MEde Groot APTen Hacken NHTKoppelman GHvan den Berge MTimens WfalseCholinergic neuroplasticity in asthma driven by TrkB signaling.Parasympathetic neurons in the airways control bronchomotor tone. Increased activity of cholinergic neurons are mediators of airway hyperresponsiveness (AHR) in asthma, however, mechanisms are not elucidated. We describe remodeling of the cholinergic neuronal network in asthmatic airways driven by brain-derived neurotrophic factor (BDNF) and Tropomyosin receptor kinase B (TrkB). Human bronchial biopsies were stained for cholinergic marker vesicular acetylcholine transporter (VAChT). Human lung gene expression and single nucleotide polymorphisms (SNP) in neuroplasticity-related genes were compared between asthma and healthy patients. Wild-type (WT) and mutated TrkB knock-in mice (Ntrk2tm1Ddg/J) with impaired BDNF signaling were chronically exposed to ovalbumin (OVA). Neuronal VAChT staining2020-01-01T00:00:00Z2020 Jun2026-04-18T01:10:52.959Z2020-09-30T07:02:08ZS-EPMC73029633227785510.1096/fj.202000170R