{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Yao Y"],"pubmed_abstract":["At present, based on whole genome sequencing, sequences and genes annotation of the sheep (Ovis aries) Y chromosome are still absent. The isolation of Y chromosomes followed by sequencing has been approved as an effective approach to analyze this complex chromosome in other species. In this study, we established a highly efficient synchronization method for G2/M phase of sheep fibroblasts, which was successfully applied to flow-sorting chromosomes of sheep, with a focus on isolation and sequencing of the ovine Y chromosome. The isolated (~80,000) Y chromosomes were verified by fluorescence quantitative real-time polymerase chain reaction, further confirmed by fluorescence in situ hybridization, and amplified by the MALBAC method before next-generation sequencing. The sequence results indic"],"journal":["Scientific reports"],"pagination":["9933"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7303189"],"repository":["biostudies-literature"],"pubmed_title":["Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting."],"pmcid":["PMC7303189"],"pubmed_authors":["Yao Y","Zhang Y","Deng X","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting.","description":"At present, based on whole genome sequencing, sequences and genes annotation of the sheep (Ovis aries) Y chromosome are still absent. The isolation of Y chromosomes followed by sequencing has been approved as an effective approach to analyze this complex chromosome in other species. In this study, we established a highly efficient synchronization method for G2/M phase of sheep fibroblasts, which was successfully applied to flow-sorting chromosomes of sheep, with a focus on isolation and sequencing of the ovine Y chromosome. The isolated (~80,000) Y chromosomes were verified by fluorescence quantitative real-time polymerase chain reaction, further confirmed by fluorescence in situ hybridization, and amplified by the MALBAC method before next-generation sequencing. The sequence results indic","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2026-04-15T13:22:32.123Z","creation":"2026-04-08T03:17:33.064Z"},"accession":"S-EPMC7303189","cross_references":{"pubmed":["32555328"],"doi":["10.1038/s41598-020-66905-x"]}}