{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Behm C"],"funding":["Austrian Science Fund FWF"],"pagination":["689-701"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7318673"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["47(6)"],"pubmed_abstract":["<h4>Aims</h4>Both, vitamin D<sub>3</sub> and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D<sub>3</sub> on the immunosuppressive properties of hPDLCs towards CD4<sup>+</sup> T lymphocytes.<h4>Material and methods</h4>Allogenic CD4<sup>+</sup> T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)<sub>2</sub> D<sub>3</sub> . After 5 days, CD4<sup>+</sup> T-lymphocyte proliferation, CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> regulatory T lymphocytes (T<sub>regs</sub> ) proportion and IL-10, TGF-β1 and IL-17A production were analysed.<h4>Results</h4"],"journal":["Journal of clinical periodontology"],"pubmed_title":["Pleiotropic effects of vitamin D<sub>3</sub> on CD4<sup>+</sup> T lymphocytes mediated by human periodontal ligament cells and inflammatory environment."],"pmcid":["PMC7318673"],"funding_grant_id":["P 29440","P29440"],"pubmed_authors":["Blufstein A","Andrukhov O","Moritz A","Gahn J","Behm C","Rausch-Fan X","Kubin B"],"additional_accession":[]},"is_claimable":false,"name":"Pleiotropic effects of vitamin D<sub>3</sub> on CD4<sup>+</sup> T lymphocytes mediated by human periodontal ligament cells and inflammatory environment.","description":"<h4>Aims</h4>Both, vitamin D<sub>3</sub> and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D<sub>3</sub> on the immunosuppressive properties of hPDLCs towards CD4<sup>+</sup> T lymphocytes.<h4>Material and methods</h4>Allogenic CD4<sup>+</sup> T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)<sub>2</sub> D<sub>3</sub> . After 5 days, CD4<sup>+</sup> T-lymphocyte proliferation, CD4<sup>+</sup> CD25<sup>+</sup> FoxP3<sup>+</sup> regulatory T lymphocytes (T<sub>regs</sub> ) proportion and IL-10, TGF-β1 and IL-17A production were analysed.<h4>Results</h4","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2025-04-27T03:50:20.005Z","creation":"2020-07-03T07:08:22Z"},"accession":"S-EPMC7318673","cross_references":{"pubmed":["32160330"],"doi":["10.1111/jcpe.13283"]}}