biostudies-literatureBehm CAustrian Science Fund FWF689-701https://www.ebi.ac.uk/biostudies/studies/S-EPMC7318673biostudies-literatureUnknown47(6)<h4>Aims</h4>Both, vitamin D₃ and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D₃ on the immunosuppressive properties of hPDLCs towards CD4⁺ T lymphocytes.<h4>Material and methods</h4>Allogenic CD4⁺ T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)₂ D₃ . After 5 days, CD4⁺ T-lymphocyte proliferation, CD4⁺ CD25⁺ FoxP3⁺ regulatory T lymphocytes (T_regs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed.<h4>Results</h4Journal of clinical periodontologyPleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment.PMC7318673P 29440P29440Blufstein AAndrukhov OMoritz AGahn JBehm CRausch-Fan XKubin BfalsePleiotropic effects of vitamin D₃ on CD4⁺ T lymphocytes mediated by human periodontal ligament cells and inflammatory environment.<h4>Aims</h4>Both, vitamin D₃ and human periodontal ligament cells (hPDLCs) possess immunosuppressive properties, but their combined effect on immune cells has never been investigated. Here, we analysed the impact of vitamin D₃ on the immunosuppressive properties of hPDLCs towards CD4⁺ T lymphocytes.<h4>Material and methods</h4>Allogenic CD4⁺ T lymphocytes were activated by phytohemagglutinin either in monoculture or co-culture with hPDLCs, in the presence or absence of IFN-γ and 1,25(OH)₂ D₃ . After 5 days, CD4⁺ T-lymphocyte proliferation, CD4⁺ CD25⁺ FoxP3⁺ regulatory T lymphocytes (T_regs ) proportion and IL-10, TGF-β1 and IL-17A production were analysed.<h4>Results</h42020-01-01T00:00:00Z2020 Jun2025-04-27T03:50:20.005Z2020-07-03T07:08:22ZS-EPMC73186733216033010.1111/jcpe.13283